Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Tyrosine Hydroxylase CRISPR/Cas9 KO Plasmid (h): sc-400273
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Tyrosine Hydroxylase CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Tyrosine Hydroxylase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Tyrosine Hydroxylase Antibody (F-11): sc-25269
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tyrosine Hydroxylase CRISPR/Cas9 KO Plasmid (h) | sc-400273 | 20 µg | $397.00 |
Overview
TH encodes tyrosine hydroxylase, the rate-limiting enzyme in catecholamine biosynthesis that catalyzes the conversion of L-tyrosine to L-DOPA, the precursor for dopamine, norepinephrine, and epinephrine. Its activity integrates tetrahydrobiopterin (BH4) cofactor availability, iron-dependent catalysis, and phosphorylation-driven regulation to control neurotransmitter output in dopaminergic and noradrenergic neurons as well as adrenal chromaffin cells. Tyrosine hydroxylase is central to neuronal differentiation, vesicular neurotransmitter loading, synaptic signaling, and cellular stress responses linked to redox balance and mitochondrial function. Dysregulation of TH expression or activity is widely studied in the context of dopamine deficiency, catecholaminergic signaling defects, and neurodegeneration-associated circuit dysfunction.
Tyrosine Hydroxylase CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TH gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TH together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TH open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Tyrosine Hydroxylase protein expression.
This CRISPR knockout system enables efficient generation of TH-deficient cell models for investigation of Tyrosine Hydroxylase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TH exon(s) critical for Tyrosine Hydroxylase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TH genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Tyrosine Hydroxylase CRISPR/Cas9 KO Plasmid (h) and Tyrosine Hydroxylase CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TH locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Tyrosine Hydroxylase HDR Plasmid (h) and Tyrosine Hydroxylase HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TH homology arms to support homology-directed repair at defined TH target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.