Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Tyrosinase Double Nickase Plasmid (m): sc-423566-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Tyrosinase Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Tyrosinase Double Nickase Plasmid (m) and Tyrosinase Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Tyr. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Tyrosinase Antibody (T311): sc-20035
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tyrosinase Double Nickase Plasmid (m) | sc-423566-NIC | 20 µg | $410.00 | |||
Tyrosinase Double Nickase Plasmid (m2) | sc-423566-NIC-2 | 20 µg | $410.00 |
Mouse Tyr encodes tyrosinase, a copper-dependent oxidase that catalyzes key rate-limiting steps in melanin biosynthesis, including the conversion of L-tyrosine to DOPA and DOPAquinone within melanosomes. Tyrosinase activity integrates with melanocyte differentiation programs and organelle biogenesis pathways that govern pigment production, trafficking, and storage. Disruption of Tyr perturbs eumelanin and pheomelanin synthesis, altering pigmentation phenotypes that are widely used as tractable readouts in developmental biology and cell biology. Because melanin pathway genes influence oxidative chemistry and cellular stress responses, Tyr is frequently studied in models of pigmentary variation and related genetic disorders.
Tyrosinase Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Tyr locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Tyr. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Tyr function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Tyr-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.