Type II 5-phosphatase CRISPR/Cas9 KO Plasmid (m): sc-421136
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Type II 5-phosphatase CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Type II 5-phosphatase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Type II 5-phosphatase CRISPR/Cas9 KO Plasmid (m) | sc-421136 | 20 µg | $397.00 |
Overview
Mouse Inpp5b encodes type II 5-phosphatase (INPP5B), an inositol polyphosphate 5-phosphatase that hydrolyzes phosphatidylinositol 4,5-bisphosphate and related phosphoinositides to shape membrane lipid signaling. By controlling local PIP2 pools, INPP5B influences phosphoinositide-dependent pathways involved in endocytosis, vesicular trafficking, and actin cytoskeleton remodeling, with downstream effects on receptor signaling and membrane dynamics. INPP5B function is closely aligned with broader PI3K/PLC signaling circuitry and intersects with ciliary and Golgi-associated trafficking processes. Dysregulated phosphoinositide turnover is widely implicated in cellular homeostasis defects, making Inpp5b a useful target for mechanistic studies of signaling and membrane transport phenotypes.
Type II 5-phosphatase CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Inpp5b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Inpp5b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Inpp5b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Type II 5-phosphatase protein expression.
This CRISPR knockout system enables efficient generation of Inpp5b-deficient cell models for investigation of Type II 5-phosphatase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Inpp5b exon(s) critical for Type II 5-phosphatase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Inpp5b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Type II 5-phosphatase CRISPR/Cas9 KO Plasmid (m) and Type II 5-phosphatase CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Inpp5b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Type II 5-phosphatase HDR Plasmid (m) and Type II 5-phosphatase HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Inpp5b homology arms to support homology-directed repair at defined Inpp5b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.