SAM Transcription Activation: SAM system utilizing dCas9-VP64, MS2-P65-HSF1, and sgRNA for precise gene transcription activation.
Type II 4-phosphatase CRISPR Activation Plasmid (h): sc-402205-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Type II 4-phosphatase CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Type II 4-phosphatase CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Type II 4-phosphatase CRISPR Activation Plasmid (h) and Type II 4-phosphatase CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the INPP4B transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Type II 4-phosphatase Antibody (3F2): sc-293234
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Type II 4-phosphatase CRISPR Activation Plasmid (h) | sc-402205-ACT | 20 µg | $397.00 |