Type I 4-phosphatase Antibody (E-2): sc-390549
- Type I 4-phosphatase Antibody (E-2) is a mouse monoclonal IgG1 κ Type I 4-phosphatase antibody provided at 200 µg/ml
- raised against amino acids 1-120 mapping at the N-terminus of Type I 4-phosphatase of human origin
- Type I 4-phosphatase Antibody (E-2) is recommended for detection of Type I 4-phosphatase of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and canine
- Anti-Type I 4-phosphatase Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Type I 4-phosphatase Antibody (E-2) for WB applications. This reagent is now offered in a bundle with Type I 4-phosphatase Antibody (E-2) (see ordering information below).
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Type I 4-phosphatase Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Type I 4-phosphatase of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Type I 4-phosphatase (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Type I 4-phosphatase plays a crucial role in cellular signaling by selectively hydrolyzing phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate, and inositol 3,4-bisphosphate, thereby generating second messengers that mediate responses to extracellular signals. This enzymatic activity is vital for regulating intracellular calcium levels, which are essential for various cellular processes, including muscle contraction, neurotransmitter release, and cell proliferation. Additionally, Type I 4-phosphatase is alternatively spliced into two isoforms, α and β, which have been identified in human platelets, rat brain, heart, skeletal muscle, and spleen, and all isoforms contain a conserved motif CKSAKDRT that includes the active site consensus sequence C-X5-R. The ability of Type I 4-phosphatase to act as a signal-terminating enzyme underscores its importance in maintaining cellular homeostasis and modulating signal transduction pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Type I 4-phosphatase Antibody (E-2) References:
- The role of phosphatases in inositol signaling reactions. | Majerus, PW., et al. 1999. J Biol Chem. 274: 10669-72. PMID: 10196133
- The isolation and characterization of inositol polyphosphate 4-phosphatase. | Bansal, VS., et al. 1990. J Biol Chem. 265: 1806-11. PMID: 2153145
- The isolation and characterization of cDNA encoding human and rat brain inositol polyphosphate 4-phosphatase. | Norris, FA., et al. 1995. J Biol Chem. 270: 16128-33. PMID: 7608176
- Inositol polyphosphate 4-phosphatase is inactivated by calpain-mediated proteolysis in stimulated human platelets. | Norris, FA., et al. 1997. J Biol Chem. 272: 10987-9. PMID: 9110986
- The cDNA cloning and characterization of inositol polyphosphate 4-phosphatase type II. Evidence for conserved alternative splicing in the 4-phosphatase family. | Norris, FA., et al. 1997. J Biol Chem. 272: 23859-64. PMID: 9295334
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Type I 4-phosphatase Antibody (E-2) | sc-390549 | 200 µg/ml | $316.00 | |||
Type I 4-phosphatase Antibody (E-2): m-IgG Fc BP-HRP Bundle | sc-526170 | 200 µg Ab; 10 µg BP | $354.00 | |||
Type I 4-phosphatase Antibody (E-2) AC | sc-390549 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Type I 4-phosphatase Antibody (E-2) HRP | sc-390549 HRP | 200 µg/ml | $316.00 | |||
Type I 4-phosphatase Antibody (E-2) FITC | sc-390549 FITC | 200 µg/ml | $330.00 | |||
Type I 4-phosphatase Antibody (E-2) PE | sc-390549 PE | 200 µg/ml | $343.00 | |||
Type I 4-phosphatase Antibody (E-2) Alexa Fluor® 488 | sc-390549 AF488 | 200 µg/ml | $357.00 | |||
Type I 4-phosphatase Antibody (E-2) Alexa Fluor® 546 | sc-390549 AF546 | 200 µg/ml | $357.00 | |||
Type I 4-phosphatase Antibody (E-2) Alexa Fluor® 594 | sc-390549 AF594 | 200 µg/ml | $357.00 | |||
Type I 4-phosphatase Antibody (E-2) Alexa Fluor® 647 | sc-390549 AF647 | 200 µg/ml | $357.00 | |||
Type I 4-phosphatase Antibody (E-2) Alexa Fluor® 680 | sc-390549 AF680 | 200 µg/ml | $357.00 | |||
Type I 4-phosphatase Antibody (E-2) Alexa Fluor® 790 | sc-390549 AF790 | 200 µg/ml | $357.00 |