Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
twist2 CRISPR/Cas9 KO Plasmid (m): sc-419989
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- twist2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the twist2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
twist2 CRISPR/Cas9 KO Plasmid (m) | sc-419989 | 20 µg | $397.00 |
Overview
Twist2 encodes a basic helix–loop–helix (bHLH) transcription factor that regulates mesenchymal lineage programs, controlling cell fate decisions, proliferation, and differentiation during embryogenesis and tissue homeostasis. In mouse, twist2 influences transcriptional networks linked to epithelial–mesenchymal transition (EMT)-like processes, extracellular matrix remodeling, and crosstalk with pathways such as TGF-β and Wnt that shape morphogenesis and stromal cell behavior. Altered TWIST2 activity has been associated with developmental abnormalities and dysregulated inflammatory and fibrotic responses, making it a useful node for studying gene regulatory circuitry in connective tissue biology. Loss-of-function models support investigation of how Twist2 coordinates transcriptional repression/activation programs in fibroblasts, myogenic precursors, and immune-associated stromal compartments.
twist2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Twist2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Twist2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Twist2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish twist2 protein expression.
This CRISPR knockout system enables efficient generation of Twist2-deficient cell models for investigation of twist2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Twist2 exon(s) critical for twist2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Twist2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by twist2 CRISPR/Cas9 KO Plasmid (m) and twist2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Twist2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by twist2 HDR Plasmid (m) and twist2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Twist2 homology arms to support homology-directed repair at defined Twist2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.