Tup1 Antibody (Clone 10): sc-58065

Tup1 Antibody (Clone 10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Tup1 protein of Saccharomyces cerevisiae origin by western blotting (WB) and immunoprecipitation (IP). anti-Tup1 antibody (Clone 10) is available as a non-conjugated format, providing researchers with a reliable tool for studying this critical protein. Tup1 plays a vital role in the regulation of gene expression, functioning as a transcriptional repressor that modulates the activity of various genes, particularly those involved in cell cycle progression and metabolic processes. Tup1 interaction with Ssn6 protein forms a general transcriptional repression complex, which is essential for maintaining cellular homeostasis and responding to environmental changes. Tup1-Ssn6 complex inhibits the expression of genes that promote cell growth under unfavorable conditions, thereby allowing yeast to conserve resources. Tup1 is involved in the recruitment of chromatin remodeling complexes, such as SNF-SWI complex, which alters chromatin structure to facilitate transcriptional activation when conditions become favorable. Understanding Tup1 function and interactions is crucial for elucidating the mechanisms of gene regulation in yeast and can provide insights into similar processes in higher eukaryotes.

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Tup1 Antibody (Clone 10) References:

1. The isolation and characterization of missense mutants in the general repressor protein Ssn6 of Saccharomyces cerevisiae.  |  Limbach, MP. and Zitomer, RS. 2000. Mol Gen Genet. 263: 455-62. PMID: 10821179
2. Evidence that SNF2/SWI2 and SNF5 activate transcription in yeast by altering chromatin structure.  |  Hirschhorn, JN., et al. 1992. Genes Dev. 6: 2288-98. PMID: 1459453
3. SNF11, a new component of the yeast SNF-SWI complex that interacts with a conserved region of SNF2.  |  Treich, I., et al. 1995. Mol Cell Biol. 15: 4240-8. PMID: 7623818
4. A role for the transcription factors Mbp1 and Swi4 in progression from G1 to S phase.  |  Koch, C., et al. 1993. Science. 261: 1551-7. PMID: 8372350
5. Switching transcription on and off during the yeast cell cycle: Cln/Cdc28 kinases activate bound transcription factor SBF (Swi4/Swi6) at start, whereas Clb/Cdc28 kinases displace it from the promoter in G2.  |  Koch, C., et al. 1996. Genes Dev. 10: 129-41. PMID: 8566747
6. Binding to the yeast SwI4,6-dependent cell cycle box, CACGAAA, is cell cycle regulated in vivo.  |  Harrington, LA. and Andrews, BJ. 1996. Nucleic Acids Res. 24: 558-65. PMID: 8604294
7. The Saccharomyces cerevisiae Start-specific transcription factor Swi4 interacts through the ankyrin repeats with the mitotic Clb2/Cdc28 kinase and through its conserved carboxy terminus with Swi6.  |  Siegmund, RF. and Nasmyth, KA. 1996. Mol Cell Biol. 16: 2647-55. PMID: 8649372
8. Dissecting the regulatory circuitry of a eukaryotic genome.  |  Holstege, FC., et al. 1998. Cell. 95: 717-28. PMID: 9845373

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tup1 Antibody (Clone 10) has 0 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tup1 Antibody (Clone 10) Specifications

Alternate Product Name: Tup1

Product Clone Name: Clone 10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-200

Epitope Target: Tup1

Epitope Species: yeast

Product Reactivity/Detection: Tup1

Species Reactivity/Detection: Saccharomyces cerevisiae

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Tup1 Antibody (Clone 10): N/A