Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TudorSN Double Nickase Plasmid (m2): sc-425184-NIC-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TudorSN Double Nickase Plasmid (m2) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TudorSN Double Nickase Plasmid (m2) and TudorSN Double Nickase Plasmid (m22) encode distinct paired gRNA designs targeting Snd1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TudorSN Antibody (F-5): sc-166676
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TudorSN Double Nickase Plasmid (m2) | sc-425184-NIC-2 | 20 µg | $410.00 |
Mouse Snd1 encodes TudorSN (staphylococcal nuclease and tudor domain containing 1), a multifunctional RNA-binding protein that integrates RNA processing with gene regulatory programs by participating in the RNA-induced silencing complex, microRNA-mediated repression, and spliceosome-associated events. TudorSN contributes to control of mRNA stability and translation and has been linked to transcriptional co-regulation, stress granule dynamics, and lipid/metabolic signaling pathways in diverse cell types. Dysregulated Snd1/TudorSN activity is associated with altered proliferative and inflammatory states and is frequently studied in the context of oncogenic signaling, immune regulation, and neurobiology. Genetic perturbation of Snd1 in mouse models supports mechanistic studies of post-transcriptional regulation, small RNA pathways, and RNA–protein interaction networks using CRISPR-based editing, functional genomics, and pathway-focused assays.
TudorSN Double Nickase Plasmid (m2) consists of a matched pair of plasmids engineered for high-specificity editing of the Snd1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Snd1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Snd1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Snd1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.