TTF/Transcription Termination Factor/TTF1 CRISPR/Cas9 KO Plasmid (m): sc-423540

Overview

Ttf1 (TTF1/Transcription Termination Factor 1) is a sequence-specific DNA-binding protein that functions as a transcription termination and replication fork barrier factor in ribosomal DNA (rDNA) repeats. In mouse cells, TTF1 helps organize nucleolar chromatin, coordinates RNA polymerase I transcription with rRNA processing, and supports genome stability by regulating rDNA replication–transcription conflicts. Through these roles, TTF1 contributes to nucleolar homeostasis and ribosome biogenesis, processes tightly linked to proliferation and cellular stress responses. Dysregulation of rDNA transcription control and nucleolar function is frequently implicated in oncogenic growth programs and broader genome instability phenotypes, making Ttf1 a relevant target in mechanistic studies.

TTF/Transcription Termination Factor/TTF1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ttf1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ttf1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ttf1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TTF/Transcription Termination Factor/TTF1 protein expression.

This CRISPR knockout system enables efficient generation of Ttf1-deficient cell models for investigation of TTF/Transcription Termination Factor/TTF1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Ttf1 exon(s) critical for TTF/Transcription Termination Factor/TTF1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Ttf1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by TTF/Transcription Termination Factor/TTF1 CRISPR/Cas9 KO Plasmid (m) and TTF/Transcription Termination Factor/TTF1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ttf1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by TTF/Transcription Termination Factor/TTF1 HDR Plasmid (m) and TTF/Transcription Termination Factor/TTF1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ttf1 homology arms to support homology-directed repair at defined Ttf1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.