TTC1 CRISPR/Cas9 KO Plasmid (m): sc-426212
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TTC1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TTC1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TTC1 CRISPR/Cas9 KO Plasmid (m) | sc-426212 | 20 µg | $397.00 |
Overview
Mouse Ttc1 encodes tetratricopeptide repeat domain protein 1 (TTC1), a TPR-containing scaffold implicated in protein–protein interactions that coordinate folding and trafficking of multi-protein complexes. TTC1 has been associated with chaperone-linked processes and regulation of signaling nodes that influence cellular stress handling, proteostasis, and cytoskeletal or organelle organization. Through these roles, TTC1 can affect cell cycle progression and differentiation programs by shaping the stability and assembly of key regulatory factors. Dysregulation of proteostasis and signaling homeostasis is broadly relevant to neurodevelopmental and neurodegenerative research contexts as well as tumor biology, making Ttc1 a useful locus for mechanistic studies.
TTC1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ttc1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ttc1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ttc1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TTC1 protein expression.
This CRISPR knockout system enables efficient generation of Ttc1-deficient cell models for investigation of TTC1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ttc1 exon(s) critical for TTC1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ttc1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TTC1 CRISPR/Cas9 KO Plasmid (m) and TTC1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ttc1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TTC1 HDR Plasmid (m) and TTC1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ttc1 homology arms to support homology-directed repair at defined Ttc1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.