TTBK1 Antibody (B-6): sc-374600
- TTBK1 Antibody (B-6) is a mouse monoclonal IgG1 κ TTBK1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 429-459 within an internal region of TTBK1 of human origin
- TTBK1 Antibody (B-6) is recommended for detection of TTBK1 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TTBK1 Antibody (B-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TTBK1 Antibody (B-6) for WB applications. These reagents are now offered in bundles with TTBK1 Antibody (B-6) (see ordering information below).
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TTBK1 Antibody (B-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects TTBK1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TTBK1 (B-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TTBK1, also known as tau tubulin kinase 1 or brain-derived tau kinase, is a 1,321 amino acid protein that features a single protein kinase domain and is classified within the serine/threonine protein kinase family. TTBK1 is primarily localized in the cytoplasm and is highly expressed in the brain, with lower expression levels found in the testis and spinal cord. TTBK1 plays a crucial role in phosphorylating Tau, a protein essential for tubulin polymerization, on threonine, tyrosine, and serine residues. The phosphorylation process, which is catalyzed by TTBK1 using divalent cations like magnesium and manganese, leads to the formation of a phosphoprotein and adenosine diphosphate (ADP). This modification of Tau is significant as it promotes Tau aggregation and results in a loss of its functional capacity, highlighting TTBK1′s important role in regulating tubulin dynamics. TTBK1 is known to interact with several other proteins, including microtubule-associated proteins, which further underscores its involvement in cellular processes related to cytoskeletal organization and neuronal function. Two isoforms of TTBK1 arise from alternative splicing, adding complexity to its regulatory mechanisms and functional diversity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TTBK1 Antibody (B-6) References:
- Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. | Nagase, T., et al. 2001. DNA Res. 8: 85-95. PMID: 11347906
- Tau-tubulin kinase 1 (TTBK1), a neuron-specific tau kinase candidate, is involved in tau phosphorylation and aggregation. | Sato, S., et al. 2006. J Neurochem. 98: 1573-84. PMID: 16923168
- Expression, purification and crystallization of a human tau-tubulin kinase 2 that phosphorylates tau protein. | Kitano-Takahashi, M., et al. 2007. Acta Crystallogr Sect F Struct Biol Cryst Commun. 63: 602-4. PMID: 17620722
- Tau is endogenously nitrated in mouse brain: identification of a tyrosine residue modified in vivo by NO. | Nonnis, S., et al. 2008. Neurochem Res. 33: 518-25. PMID: 17768677
- A novel tau-tubulin kinase from bovine brain. | Takahashi, M., et al. 1995. FEBS Lett. 372: 59-64. PMID: 7556643
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TTBK1 Antibody (B-6) | sc-374600 | 200 µg/ml | $316.00 | |||
TTBK1 Antibody (B-6): m-IgG Fc BP-HRP Bundle | sc-527489 | 200 µg Ab; 10 µg BP | $354.00 | |||
TTBK1 Antibody (B-6): m-IgG1 BP-HRP Bundle | sc-532862 | 200 µg Ab; 20 µg BP | $354.00 | |||
TTBK1 Antibody (B-6) AC | sc-374600 AC | 500 µg/ml, 25% agarose | $416.00 | |||
TTBK1 Antibody (B-6) HRP | sc-374600 HRP | 200 µg/ml | $316.00 | |||
TTBK1 Antibody (B-6) FITC | sc-374600 FITC | 200 µg/ml | $330.00 | |||
TTBK1 Antibody (B-6) PE | sc-374600 PE | 200 µg/ml | $343.00 | |||
TTBK1 Antibody (B-6) Alexa Fluor® 488 | sc-374600 AF488 | 200 µg/ml | $357.00 | |||
TTBK1 Antibody (B-6) Alexa Fluor® 546 | sc-374600 AF546 | 200 µg/ml | $357.00 | |||
TTBK1 Antibody (B-6) Alexa Fluor® 594 | sc-374600 AF594 | 200 µg/ml | $357.00 | |||
TTBK1 Antibody (B-6) Alexa Fluor® 647 | sc-374600 AF647 | 200 µg/ml | $357.00 | |||
TTBK1 Antibody (B-6) Alexa Fluor® 680 | sc-374600 AF680 | 200 µg/ml | $357.00 | |||
TTBK1 Antibody (B-6) Alexa Fluor® 790 | sc-374600 AF790 | 200 µg/ml | $357.00 | |||
TTBK1 (B-6) Neutralizing Peptide | sc-374600 P | 100 µg/0.5 ml | $68.00 |