TSPO2 CRISPR/Cas9 KO Plasmid (h): sc-406342
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TSPO2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TSPO2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TSPO2 CRISPR/Cas9 KO Plasmid (h) | sc-406342 | 20 µg | $397.00 |
Overview
TSPO2 (translocator protein 2) is a mitochondria-associated, TSPO family member expressed predominantly in erythroid lineage cells, where it supports terminal erythropoiesis and red blood cell maturation. It has been implicated in regulating mitochondrial membrane organization and metabolite/porphyrin handling, linking TSPO2 activity to heme synthesis and erythroid differentiation programs. Through these processes, TSPO2 influences cellular redox balance and mitochondrial function during reticulocyte remodeling. Altered TSPO2 expression or function has been investigated in the context of erythroid disorders and anemia-related phenotypes, making it a useful target for mechanistic studies in hematopoietic systems.
TSPO2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TSPO2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TSPO2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TSPO2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TSPO2 protein expression.
This CRISPR knockout system enables efficient generation of TSPO2-deficient cell models for investigation of TSPO2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TSPO2 exon(s) critical for TSPO2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TSPO2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TSPO2 CRISPR/Cas9 KO Plasmid (h) and TSPO2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TSPO2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TSPO2 HDR Plasmid (h) and TSPO2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TSPO2 homology arms to support homology-directed repair at defined TSPO2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.