TSPAN7 CRISPR/Cas9 KO Plasmid (m): sc-423425
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TSPAN7 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TSPAN7 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TSPAN7 CRISPR/Cas9 KO Plasmid (m) | sc-423425 | 20 µg | $397.00 |
Overview
Tspan7 encodes the tetraspanin protein TSPAN7, a multi-pass membrane scaffold that organizes tetraspanin-enriched microdomains and modulates the distribution and signaling of partner receptors at the cell surface. In mouse, TSPAN7 is implicated in membrane trafficking, cytoskeletal remodeling, and cell–cell communication processes that influence neuronal development and synaptic organization, and it can also affect adhesion and motility programs in other cell types. Through interactions that shape receptor clustering and downstream signaling, TSPAN7 contributes to pathways linked to endocytosis, actin dynamics, and signal transduction at specialized membrane compartments. Dysregulated tetraspanin network function has been associated with neurodevelopmental phenotypes and altered cellular connectivity, making Tspan7 a useful locus for mechanistic studies of membrane microdomain biology.
TSPAN7 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tspan7 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tspan7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tspan7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TSPAN7 protein expression.
This CRISPR knockout system enables efficient generation of Tspan7-deficient cell models for investigation of TSPAN7 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tspan7 exon(s) critical for TSPAN7 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tspan7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TSPAN7 CRISPR/Cas9 KO Plasmid (m) and TSPAN7 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tspan7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TSPAN7 HDR Plasmid (m) and TSPAN7 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tspan7 homology arms to support homology-directed repair at defined Tspan7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.