TSPAN18 CRISPR/Cas9 KO Plasmid (h): sc-405937
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TSPAN18 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TSPAN18 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TSPAN18 CRISPR/Cas9 KO Plasmid (h) | sc-405937 | 20 µg | $397.00 |
Overview
TSPAN18 encodes a tetraspanin family membrane protein that helps organize tetraspanin-enriched microdomains, coordinating lateral interactions among receptors, adhesion molecules, and signaling adaptors at the plasma membrane. Through these scaffolding functions, TSPAN18 can influence processes such as cell–cell communication, membrane trafficking, and signal integration that shape cellular activation and motility programs. Expression and genetic variation in tetraspanins, including TSPAN18, have been linked in the literature to platelet-related phenotypes and vascular biology, supporting use of this target in studies of hemostasis-associated signaling and inflammatory responses. Dissecting TSPAN18 function can clarify how membrane microdomain composition modulates downstream pathways relevant to cardiovascular and immune-associated disease mechanisms.
TSPAN18 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TSPAN18 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TSPAN18 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TSPAN18 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TSPAN18 protein expression.
This CRISPR knockout system enables efficient generation of TSPAN18-deficient cell models for investigation of TSPAN18 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TSPAN18 exon(s) critical for TSPAN18 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TSPAN18 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TSPAN18 CRISPR/Cas9 KO Plasmid (h) and TSPAN18 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TSPAN18 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TSPAN18 HDR Plasmid (h) and TSPAN18 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TSPAN18 homology arms to support homology-directed repair at defined TSPAN18 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.