TSHβ Antibody (D-6): sc-365801

TSHβ Antibody (D-6) is a mouse monoclonal IgG3 kappa light chain antibody that detects TSH beta of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-TSH beta antibody (D-6) is available as the non-conjugated form. TSH beta plays a crucial role in the endocrine system as a component of thyroid-stimulating hormone (TSH), which regulates thyroid gland function and metabolism. TSH is a glycoprotein that stimulates thyroid hormone release, influencing growth, development, and metabolism. TSH beta regulation occurs through thyroid hormones, particularly triiodothyronine (T3), and retinoid compounds, maintaining balanced thyroid hormone levels. TSH beta interacts with thyroid-stimulating hormone receptor (TSHR), which exists in multiple glycosylation states affecting activity. TSHR′s third cytoplasmic loop mediates intracellular signaling pathways, including inositol phosphate and cyclic adenosine monophosphate (cAMP) formation, demonstrating complex thyroid function regulation.

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TSHβ Antibody (D-6) References:

1. Ligand-induced recruitment of a histone deacetylase in the negative-feedback regulation of the thyrotropin beta gene.  |  Sasaki, S., et al. 1999. EMBO J. 18: 5389-98. PMID: 10508171
2. Cloning of bullfrog thyroid-stimulating hormone (TSH) beta subunit cDNA: expression of TSHbeta mRNA during metamorphosis.  |  Okada, R., et al. 2000. Gen Comp Endocrinol. 119: 224-31. PMID: 10936043
3. Thyroid-hormone-dependent negative regulation of thyrotropin beta gene by thyroid hormone receptors: study with a new experimental system using CV1 cells.  |  Nakano, K., et al. 2004. Biochem J. 378: 549-57. PMID: 14611644
4. Differential expression of thyroid-stimulating hormone beta subunit in gonads during sex reversal of orange-spotted and red-spotted groupers.  |  Wang, Y., et al. 2004. Mol Cell Endocrinol. 220: 77-88. PMID: 15196702
5. A novel thyroid stimulating hormone beta-subunit isoform in human pituitary, peripheral blood leukocytes, and thyroid.  |  Schaefer, JS. and Klein, JR. 2009. Gen Comp Endocrinol. 162: 241-4. PMID: 19364510
6. Thyroid stimulating hormone β-subunit splice variant is expressed in all fractional subsets of bone marrow hematopoietic cells and peripheral blood leukocytes and is modulated during bacterial infection.  |  Weber, A., et al. 2020. Gen Comp Endocrinol. 294: 113495. PMID: 32360559
7. Immunochemical characterization of two thyroid-stimulating hormone beta-subunit epitopes.  |  Fairlie, WD., et al. 1995. Biochem J. 308 (Pt 1): 203-10. PMID: 7538754
8. Substitutions of different regions of the third cytoplasmic loop of the thyrotropin (TSH) receptor have selective effects on constitutive, TSH-, and TSH receptor autoantibody-stimulated phosphoinositide and 3',5'-cyclic adenosine monophosphate signal generation.  |  Kosugi, S., et al. 1993. Mol Endocrinol. 7: 1009-20. PMID: 7901757
9. Multimeric complex formation by the thyrotropin receptor in solubilized thyroid membranes.  |  Graves, PN., et al. 1996. Endocrinology. 137: 3915-20. PMID: 8756566
10. The rat TSHbeta gene contains distinct response elements for regulation by retinoids and thyroid hormone.  |  Breen, JJ., et al. 1997. Mol Cell Endocrinol. 131: 137-46. PMID: 9296372
11. Understanding the thyrotropin receptor function-structure relationship.  |  Sanders, J., et al. 1997. Baillieres Clin Endocrinol Metab. 11: 451-79. PMID: 9532334
12. Functional homodimeric glycoprotein hormones: implications for hormone action and evolution.  |  Moyle, WR., et al. 1998. Chem Biol. 5: 241-54. PMID: 9646940

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TSHβ Antibody (D-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TSHβ Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TSHβ Antibody (D-6) Specifications

Alternate Product Name: TSHβ

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 101-131

Epitope Target: TSHβ

Epitope Species: human

Product Reactivity/Detection: TSHβ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TSHβ Antibody (D-6): P: Blocking Peptide