TSEN54 Antibody (C-1): sc-374488
- TSEN54 Antibody (C-1) is a mouse monoclonal IgG1 κ TSEN54 antibody provided at 200 µg/ml
- raised against amino acids 11-142 mapping near the N-terminus of TSEN54 of human origin
- TSEN54 Antibody (C-1) is recommended for detection of TSEN54 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TSEN54 Antibody (C-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-374488 X, 200 µg/0.1 ml)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TSEN54 Antibody (C-1). This work is in progress.
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TSEN54 Antibody (C-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects TSEN54 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TSEN54 (C-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TSEN54, also known as tRNA splicing endonuclease 54 homolog, plays a crucial role in the tRNA-splicing endonuclease complex, which is essential for the accurate processing of pre-tRNA by identifying and cleaving introns at both the 5′ and 3′ splice sites. This function ensures the production of mature tRNA molecules necessary for protein synthesis. TSEN54 is involved in mRNA processing, linking pre-tRNA and pre-mRNA splicing events through interactions with pre-mRNA processing factors. TSEN54 consists of 526 amino acids and is localized in the nucleus, where TSEN54 forms a heterotetrameric complex integral to the splicing process. Defects in TSEN54 have been associated with pontocerebellar hypoplasia (PCH) types 4 and 2A, conditions characterized by significant structural abnormalities in the cerebellum, inferior olive, and ventral pons. TSEN54 exists in two alternatively spliced isoforms, highlighting TSEN54′s complexity and importance in cellular function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TSEN54 Antibody (C-1) References:
- Identification of a human endonuclease complex reveals a link between tRNA splicing and pre-mRNA 3' end formation. | Paushkin, SV., et al. 2004. Cell. 117: 311-21. PMID: 15109492
- DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage. | Zody, MC., et al. 2006. Nature. 440: 1045-9. PMID: 16625196
- tRNA splicing endonuclease mutations cause pontocerebellar hypoplasia. | Budde, BS., et al. 2008. Nat Genet. 40: 1113-8. PMID: 18711368
- Clinical, neuroradiological and genetic findings in pontocerebellar hypoplasia. | Namavar, Y., et al. 2011. Brain. 134: 143-56. PMID: 20952379
- Pontocerebellar hypoplasia: clinical, pathologic, and genetic studies. | Cassandrini, D., et al. 2010. Neurology. 75: 1459-64. PMID: 20956791
- Impairment of the tRNA-splicing endonuclease subunit 54 (tsen54) gene causes neurological abnormalities and larval death in zebrafish models of pontocerebellar hypoplasia. | Kasher, PR., et al. 2011. Hum Mol Genet. 20: 1574-84. PMID: 21273289
- TSEN54 mutations cause pontocerebellar hypoplasia type 5. | Namavar, Y., et al. 2011. Eur J Hum Genet. 19: 724-6. PMID: 21368912
- Pontocerebellar hypoplasia: review of classification and genetics, and exclusion of several genes known to be important for cerebellar development. | Maricich, SM., et al. 2011. J Child Neurol. 26: 288-94. PMID: 21383226
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TSEN54 Antibody (C-1) | sc-374488 | 200 µg/ml | $316.00 | |||
TSEN54 Antibody (C-1) X | sc-374488 X | 200 µg/0.1 ml | $316.00 | |||
TSEN54 Antibody (C-1) AC | sc-374488 AC | 500 µg/ml, 25% agarose | $416.00 | |||
TSEN54 Antibody (C-1) HRP | sc-374488 HRP | 200 µg/ml | $316.00 | |||
TSEN54 Antibody (C-1) FITC | sc-374488 FITC | 200 µg/ml | $330.00 | |||
TSEN54 Antibody (C-1) PE | sc-374488 PE | 200 µg/ml | $343.00 | |||
TSEN54 Antibody (C-1) Alexa Fluor® 488 | sc-374488 AF488 | 200 µg/ml | $357.00 | |||
TSEN54 Antibody (C-1) Alexa Fluor® 546 | sc-374488 AF546 | 200 µg/ml | $357.00 | |||
TSEN54 Antibody (C-1) Alexa Fluor® 594 | sc-374488 AF594 | 200 µg/ml | $357.00 | |||
TSEN54 Antibody (C-1) Alexa Fluor® 647 | sc-374488 AF647 | 200 µg/ml | $357.00 | |||
TSEN54 Antibody (C-1) Alexa Fluor® 680 | sc-374488 AF680 | 200 µg/ml | $357.00 | |||
TSEN54 Antibody (C-1) Alexa Fluor® 790 | sc-374488 AF790 | 200 µg/ml | $357.00 |