TSC-22 Antibody (R5-6): sc-101195

TSC-22 Antibody (R5-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects TSC-22 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-TSC-22 antibody (R5-6) is available as the non-conjugated format. TSC-22, also known as Transforming Growth Factor Beta-Stimulated Clone-22, plays a crucial role as a transcriptional regulator, influencing cell growth, differentiation, and apoptosis. Notably, TSC-22 contains a leucine zipper domain and a nuclear export signal, which facilitate localization in the cytoplasm under normal conditions. However, during apoptosis, TSC-22 translocates to the nucleus, where TSC-22 exerts transcriptional regulatory functions. This dynamic localization underscores TSC-22′s role in the TGF-beta signaling pathway and the peroxisome proliferator-activated receptor gamma (PPARγ) pathway, both implicated in tumor suppression. Downregulation of TSC-22 mRNA in various cancers, including brain and salivary gland tumors, highlights TSC-22′s potential as a critical player in inhibiting cell proliferation and suggests importance in cancer research and therapeutic strategies.

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TSC-22 Antibody (R5-6) References:

1. Nuclear translocation of Tsc-22 (TGF-beta-stimulated clone-22) concomitant with apoptosis: Tsc-22 as a putative transcriptional regulator.  |  Hino, S., et al. 2000. Biochem Biophys Res Commun. 278: 659-64. PMID: 11095965
2. Leucine zipper structure of Tsc-22 (TGF-beta stimulated clone-22) markedly inhibits the anchorage-independent growth of salivary gland cancer cells.  |  Hino, S., et al. 2002. Oncol Rep. 9: 371-4. PMID: 11836610
3. Cytoplasmic Tsc-22 (transforming growth factor-beta-stimulated clone-22) markedly enhances the radiation sensitivity of salivary gland cancer cells.  |  Hino, S., et al. 2002. Biochem Biophys Res Commun. 292: 957-63. PMID: 11944908
4. Peroxisome proliferator-activated receptor gamma and transforming growth factor-beta pathways inhibit intestinal epithelial cell growth by regulating levels of Tsc-22.  |  Gupta, RA., et al. 2003. J Biol Chem. 278: 7431-8. PMID: 12468551
5. Downregulation of putative tumor suppressor gene Tsc-22 in human brain tumors.  |  Shostak, KO., et al. 2003. J Surg Oncol. 82: 57-64. PMID: 12501169
6. Posttranscriptional regulation of Tsc-22 (TGF-beta-stimulated clone-22) gene by TGF-beta 1.  |  Uchida, D., et al. 2003. Biochem Biophys Res Commun. 305: 846-54. PMID: 12767908
7. Tsc-22 (TGF-beta stimulated clone-22): a novel molecular target for differentiation-inducing therapy in salivary gland cancer.  |  Kawamata, H., et al. 2004. Curr Cancer Drug Targets. 4: 521-9. PMID: 15379637
8. Tsc-22 enhances TGF-beta signaling by associating with Smad4 and induces erythroid cell differentiation.  |  Choi, SJ., et al. 2005. Mol Cell Biochem. 271: 23-8. PMID: 15881652
9. Tsc-22 promotes transforming growth factor β-mediated cardiac myofibroblast differentiation by antagonizing Smad7 activity.  |  Yan, X., et al. 2011. Mol Cell Biol. 31: 3700-9. PMID: 21791611
10. Crucial role of Tsc-22 in preventing the proteasomal degradation of p53 in cervical cancer.  |  Yoon, CH., et al. 2012. PLoS One. 7: e42006. PMID: 22870275
11. Tsc-22 Promotes Interleukin-2-Deprivation Induced Apoptosis in T-Lymphocytes.  |  Pépin, A., et al. 2016. J Cell Biochem. 117: 1855-68. PMID: 26752201
12. Tsc-22 inhibits CSF-1R function and induces apoptosis in cervical cancer.  |  Cho, MJ., et al. 2017. Oncotarget. 8: 97990-98003. PMID: 29228668

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TSC-22 Antibody (R5-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TSC-22 Antibody (R5-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TSC-22 Antibody (R5-6) Specifications

Alternate Product Name: TSC22D1

Product Clone Name: R5-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-145

Epitope Target: TSC-22

Epitope Species: human

Product Reactivity/Detection: TSC-22

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TSC-22 Antibody (R5-6): N/A

The preferred GILZ antibody is GILZ Antibody (G-5): sc-133215.