Trypsin-3 CRISPR/Cas9 KO Plasmid (m): sc-423520
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Trypsin-3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Trypsin-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Trypsin-3 CRISPR/Cas9 KO Plasmid (m) | sc-423520 | 20 µg | $397.00 |
Overview
Prss3 encodes trypsin-3, a secreted serine protease that catalyzes proteolytic cleavage after lysine or arginine residues and contributes to extracellular protein turnover. In mouse tissues, trypsin-3 activity can influence pericellular remodeling by processing protease-activated receptor (PAR) signaling components and interacting with endogenous inhibitors such as serine protease inhibitors, shaping inflammatory and barrier-related responses. Dysregulated trypsin-family proteolysis has been linked to aberrant epithelial dynamics, pancreatitis-associated pathways, and tumor-associated microenvironment remodeling through changes in cell adhesion and matrix processing. As a result, Prss3 is studied for its roles in protease networks that regulate secretion, tissue homeostasis, and protease-driven signaling cascades.
Trypsin-3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Prss3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Prss3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Prss3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Trypsin-3 protein expression.
This CRISPR knockout system enables efficient generation of Prss3-deficient cell models for investigation of Trypsin-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Prss3 exon(s) critical for Trypsin-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Prss3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Trypsin-3 CRISPR/Cas9 KO Plasmid (m) and Trypsin-3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Prss3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Trypsin-3 HDR Plasmid (m) and Trypsin-3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Prss3 homology arms to support homology-directed repair at defined Prss3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.