Trx Antibody (Trx1): sc-13526

Trx Antibody (Trx1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Thioredoxin (Trx) of E. coli origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Trx antibody (Trx1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Trx is a crucial redox protein that plays a significant role in cellular processes across various species, including bacteria, plants, and mammals. Trx contains a conserved active site characterized by the sequence Trp-Cys-Gly-Pro-Cys, which is essential for function. Trx serves as a hydrogen donor for ribonucleotide reductase, an enzyme critical for DNA synthesis, maintaining redox balance within cells. This function influences cell proliferation and survival, impacting processes such as growth and apoptosis. Trx modulates DNA-binding activity of several transcription factors, including NFkB, AP-1, p53, TFIIIC, and glucocorticoid receptor, which are integral to various signaling pathways. Trx involvement in these pathways highlights potential roles in human diseases, including cancer, where inhibition has been associated with antitumor activity. Trx interacts with Thioredoxin Reductase (TrxR), a flavoprotein that catalyzes NADPH-dependent reduction of oxidized cellular components, underscoring importance in cellular redox homeostasis and therapeutic strategies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Trx Antibody (Trx1) References:

1. Redox regulation of stress signals: possible roles of dendritic stellate TRX producer cells (DST cell types).  |  Yodoi, J., et al. 2002. Biol Chem. 383: 585-90. PMID: 12033447
2. Direct association of thioredoxin-1 (TRX) with macrophage migration inhibitory factor (MIF): regulatory role of TRX on MIF internalization and signaling.  |  Son, A., et al. 2009. Antioxid Redox Signal. 11: 2595-605. PMID: 19601712
3. Nitrosative/oxidative stress conditions regulate thioredoxin-interacting protein (TXNIP) expression and thioredoxin-1 (TRX-1) nuclear localization.  |  Ogata, FT., et al. 2013. PLoS One. 8: e84588. PMID: 24376827
4. Recombinant expressed vector pET32a (+) S constructed by ligation independent cloning.  |  Wang, Y., et al. 2014. Molecules. 19: 16179-89. PMID: 25310147
5. Thioredoxin priming prolongs lung allograft survival by promoting immune tolerance.  |  Hu, H., et al. 2015. PLoS One. 10: e0124705. PMID: 25933390
6. Thioredoxin induces Tregs to generate an immunotolerant tumor microenvironment in metastatic melanoma.  |  Wang, X., et al. 2015. Oncoimmunology. 4: e1027471. PMID: 26405597
7. An improved conjugate vaccine technology; induction of antibody responses to the tumor vasculature.  |  Huijbers, EJM., et al. 2018. Vaccine. 36: 3054-3060. PMID: 29655625
8. Inhibition of Human Corneal Myofibroblast Formation.  |  Guo, X., et al. 2018. Invest Ophthalmol Vis Sci. 59: 3511-3520. PMID: 30025094
9. Catalytic Cleavage of Disulfide Bonds in Small Molecules and Linkers of Antibody-Drug Conjugates.  |  Zhang, D., et al. 2019. Drug Metab Dispos. 47: 1156-1163. PMID: 31085544
10. Identification and characterization of Eimeria tenella EtTrx1 protein.  |  Li, H., et al. 2022. Vet Parasitol. 310: 109785. PMID: 35994916

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Trx Antibody (Trx1) has 8 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Trx Antibody (Trx1) Specifications

Alternate Product Name: Trx

Product Clone Name: Trx1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Trx

Epitope Species: E. coli

Product Reactivity/Detection: Trx and Trx fusion proteins

Species Reactivity/Detection: E. coli

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for Trx Antibody (Trx1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Trx antibody is Trx Antibody (D-4): sc-271281.