Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Troponin T-C CRISPR/Cas9 KO Plasmid (h): sc-400949
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Troponin T-C CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Troponin T-C genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Troponin T-C Antibody (CT3): sc-20025
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Troponin T-C CRISPR/Cas9 KO Plasmid (h) | sc-400949 | 20 µg | $397.00 |
Overview
TNNT2 encodes cardiac troponin T, a core component of the troponin complex that couples Ca²⁺ signaling to thin-filament regulation of actin–myosin interaction in striated muscle. By anchoring the troponin complex to tropomyosin, TNNT2 helps coordinate sarcomere contraction dynamics, myofibrillar organization, and excitation–contraction coupling within cardiomyocytes. Genetic variation or dysregulated expression of TNNT2 is linked to inherited cardiomyopathies and contractile dysfunction, making it a key molecular entry point for studying sarcomeric biology, calcium-handling networks, and stress-response pathways in human cardiac cells. TNNT2 is therefore widely used as a functional node for modeling disease-associated remodeling, assessing myofilament sensitivity, and benchmarking cardiomyocyte maturation in vitro.
Troponin T-C CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TNNT2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TNNT2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TNNT2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Troponin T-C protein expression.
This CRISPR knockout system enables efficient generation of TNNT2-deficient cell models for investigation of Troponin T-C signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TNNT2 exon(s) critical for Troponin T-C function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TNNT2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Troponin T-C CRISPR/Cas9 KO Plasmid (h) and Troponin T-C CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TNNT2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Troponin T-C HDR Plasmid (h) and Troponin T-C HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TNNT2 homology arms to support homology-directed repair at defined TNNT2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.