Troponin I-SS CRISPR/Cas9 KO Plasmid (m): sc-423457

Overview

Tnni1 encodes the slow skeletal muscle isoform of troponin I (Troponin I-SS), an inhibitory subunit of the troponin complex that regulates actin–myosin interaction in a Ca²⁺-dependent manner. By modulating thin-filament responsiveness to Ca²⁺ through interactions with troponin C, troponin T, and tropomyosin, Troponin I-SS helps set contraction kinetics and relaxation properties in oxidative, slow-twitch fibers. Tnni1 expression and isoform switching are tightly linked to myofiber maturation, neuromuscular activity, and metabolic adaptation pathways that shape muscle performance and endurance. Dysregulation of troponin complex composition and sarcomeric Ca²⁺ handling is relevant to studies of muscle weakness, myopathies, and remodeling in aging or disuse, where altered contractile regulation contributes to functional decline.

Troponin I-SS CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tnni1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tnni1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tnni1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Troponin I-SS protein expression.

This CRISPR knockout system enables efficient generation of Tnni1-deficient cell models for investigation of Troponin I-SS signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Tnni1 exon(s) critical for Troponin I-SS function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Tnni1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Troponin I-SS CRISPR/Cas9 KO Plasmid (m) and Troponin I-SS CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tnni1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Troponin I-SS HDR Plasmid (m) and Troponin I-SS HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tnni1 homology arms to support homology-directed repair at defined Tnni1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.