Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Troponin I-C CRISPR/Cas9 KO Plasmid (m): sc-423459
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Troponin I-C CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Troponin I-C genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Troponin I Antibody (E-9): sc-365446
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Troponin I-C CRISPR/Cas9 KO Plasmid (m) | sc-423459 | 20 µg | $397.00 |
Overview
Tnni3 encodes the cardiac troponin I isoform that forms a regulatory complex with troponin T and troponin C on the thin filament to control actin–myosin interaction during excitation–contraction coupling. Through Ca²⁺-dependent modulation of myosin ATPase activity, troponin I helps tune sarcomere contractility and relaxation, integrating signaling inputs such as kinase-mediated phosphorylation that alters myofilament Ca²⁺ sensitivity. In mouse cardiomyocytes, Tnni3 is central to sarcomere organization and calcium handling processes that underpin cardiac output. Altered troponin I function or regulation is commonly studied in the context of cardiomyopathy mechanisms, contractile dysfunction, and stress-induced remodeling phenotypes.
Troponin I-C CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tnni3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tnni3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tnni3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Troponin I-C protein expression.
This CRISPR knockout system enables efficient generation of Tnni3-deficient cell models for investigation of Troponin I-C signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tnni3 exon(s) critical for Troponin I-C function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tnni3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Troponin I-C CRISPR/Cas9 KO Plasmid (m) and Troponin I-C CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tnni3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Troponin I-C HDR Plasmid (m) and Troponin I-C HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tnni3 homology arms to support homology-directed repair at defined Tnni3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.