TrkB Double Nickase Plasmid (h): sc-400142-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TrkB Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TrkB Double Nickase Plasmid (h) and TrkB Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NTRK2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TrkB Antibody (H-8): sc-136990
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TrkB Double Nickase Plasmid (h) | sc-400142-NIC | 20 µg | $410.00 | |||
TrkB Double Nickase Plasmid (h2) | sc-400142-NIC-2 | 20 µg | $410.00 |
NTRK2 encodes the receptor tyrosine kinase TrkB, a high-affinity receptor for BDNF and NT-4/5 that regulates neuronal differentiation, synaptic plasticity, and activity-dependent survival. Ligand binding induces TrkB autophosphorylation and engages PI3K–AKT, RAS–MAPK/ERK, and PLCγ signaling to control transcriptional programs, cytoskeletal remodeling, and calcium-dependent processes. In human biology, dysregulated TrkB signaling has been implicated in neurodevelopmental and neuropsychiatric phenotypes and is also studied in oncogenic contexts where altered receptor activity can affect proliferation and migration. As a pathway node integrating neurotrophin cues with growth and stress responses, TrkB is widely used in mechanistic studies of circuit function, cell fate decisions, and signal transduction.
TrkB Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NTRK2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NTRK2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NTRK2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NTRK2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.