TrkB (h): 293T Lysate: sc-113925

Trk B (h): 293T Lysate is derived from the human embryonic kidney 293T cell line, which has been genetically engineered to express the human tropomyosin receptor kinase B (TrkB). TrkB is a receptor tyrosine kinase essential for brain development and function, primarily through its role in mediating the effects of brain-derived neurotrophic factor (BDNF) and other neurotrophins. The 293T cell line, known for its high transfection efficiency and robust protein production, is ideal for producing TrkB lysates. In research, Trk B (h): 293T Lysate is utilized to study the molecular mechanisms of neurotrophin signaling and its impact on neuronal survival, differentiation, and synaptic plasticity. Upon binding to BDNF, TrkB undergoes dimerization and autophosphorylation, activating downstream signaling pathways such as the PI3K/Akt, MAPK/ERK, and PLCγ pathways. Researchers use this lysate in various biochemical assays, including Western blotting, immunoprecipitation, and kinase assays, to analyze TrkB expression, phosphorylation status, and interaction with downstream signaling molecules. Additionally, Trk B (h): 293T Lysate is valuable in functional studies to explore the role of TrkB in neuronal development and synaptic modulation. This lysate aids in investigating the effects of TrkB activation on neuronal growth, differentiation, and survival, providing insights into the signaling networks that regulate brain function and development. Through these studies, researchers gain a deeper understanding of the mechanisms by which TrkB mediates neurotrophic effects, advancing knowledge in neurobiology and cellular signaling.