Trk A Double Nickase Plasmid (h): sc-400188-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Trk A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Trk A Double Nickase Plasmid (h) and Trk A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NTRK1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Trk A Antibody (Y32Ex): sc-80398
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Trk A Double Nickase Plasmid (h) | sc-400188-NIC | 20 µg | $410.00 | |||
Trk A Double Nickase Plasmid (h2) | sc-400188-NIC-2 | 20 µg | $410.00 |
NTRK1 encodes the human Trk A receptor tyrosine kinase, the high-affinity receptor for nerve growth factor (NGF) that regulates neuronal survival, differentiation, and axon growth. Ligand-induced Trk A dimerization and autophosphorylation engages downstream signaling through MAPK/ERK, PI3K/AKT, and PLCγ pathways to coordinate transcriptional programs and calcium-dependent processes. NTRK1 dysfunction is linked to altered sensory and sympathetic neuron development and is implicated in neurodevelopmental disorders, while aberrant Trk signaling can contribute to oncogenic pathway activation in disease-relevant cellular contexts. As a result, NTRK1 is widely studied in neurobiology, signal transduction, and models examining growth factor–dependent phenotypes.
Trk A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NTRK1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NTRK1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NTRK1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NTRK1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.