Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TRIP6 CRISPR/Cas9 KO Plasmid (m): sc-423507
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRIP6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TRIP6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TRIP6 Antibody (F-8): sc-166310
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRIP6 CRISPR/Cas9 KO Plasmid (m) | sc-423507 | 20 µg | $397.00 |
Overview
Trip6 encodes TRIP6, a LIM domain–containing adaptor protein that localizes to focal adhesions and the actin cytoskeleton to coordinate integrin signaling, cytoskeletal remodeling, and transcriptional responses. TRIP6 functions as a scaffold for kinases and signaling complexes, linking Rho family GTPase pathways and focal adhesion turnover to regulation of cell adhesion, spreading, and migration. Through interactions with cytoskeletal and nuclear partners, it contributes to mechanotransduction and modulation of gene expression programs that shape cellular responses to extracellular matrix cues. Dysregulation of TRIP6-associated adhesion and motility networks is relevant to studying invasive phenotypes and tissue remodeling processes in disease-relevant models.
TRIP6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Trip6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Trip6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Trip6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TRIP6 protein expression.
This CRISPR knockout system enables efficient generation of Trip6-deficient cell models for investigation of TRIP6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Trip6 exon(s) critical for TRIP6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Trip6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TRIP6 CRISPR/Cas9 KO Plasmid (m) and TRIP6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Trip6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TRIP6 HDR Plasmid (m) and TRIP6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Trip6 homology arms to support homology-directed repair at defined Trip6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.