Trimethyl Histone H3 Antibody (6F12-H4): sc-130356

Trimethyl Histone H3 Antibody (6F12-H4) is a mouse monoclonal IgG1 antibody that detects Lys 9 trimethylated Histone H3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Trimethyl Histone H3 (6F12-H4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Lys 9 trimethylated Histone H3 plays a crucial role in regulating gene expression and chromatin structure as a key component of nucleosomes, which package DNA into a compact, organized form. Trimethylation of Lys 9 on Histone H3 is associated with transcriptional repression and serves as a marker for heterochromatin, essential for maintaining genome stability and regulating DNA access for transcription and replication. Understanding trimethylation dynamics helps elucidate epigenetic regulation mechanisms and their implications in development, differentiation, and disease states.

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Trimethyl Histone H3 Antibody (6F12-H4) References:

1. The transcriptional repressor JHDM3A demethylates trimethyl histone H3 lysine 9 and lysine 36.  |  Klose, RJ., et al. 2006. Nature. 442: 312-6. PMID: 16732292
2. A minimal CENP-A core is required for nucleation and maintenance of a functional human centromere.  |  Okamoto, Y., et al. 2007. EMBO J. 26: 1279-91. PMID: 17318187
3. Widespread, exceptionally high levels of histone H3 lysine 4 trimethylation largely mediate 'privileged' gene expression.  |  Chen, L., et al. 2007. Gene Expr. 13: 271-82. PMID: 17605300
4. Methylation of histone H3 lysine 27 associated with apoptosis in osteosarcoma cells induced by staurosporine.  |  Cheng, MF., et al. 2009. Histol Histopathol. 24: 1105-11. PMID: 19609857
5. DNA binding sites target nuclear NFATc1 to heterochromatin regions in adult skeletal muscle fibers.  |  Shen, T., et al. 2010. Histochem Cell Biol. 134: 387-402. PMID: 20865272
6. The CpG island shore of the GLT-1 gene acts as a methylation-sensitive enhancer.  |  Perisic, T., et al. 2012. Glia. 60: 1345-55. PMID: 22593010
7. Development of multiple cell-based assays for the detection of histone H3 Lys27 trimethylation (H3K27me3).  |  Qian, J., et al. 2013. Assay Drug Dev Technol. 11: 449-56. PMID: 23992119
8. Inositol pyrophosphates regulate JMJD2C-dependent histone demethylation.  |  Burton, A., et al. 2013. Proc Natl Acad Sci U S A. 110: 18970-5. PMID: 24191012
9. SUV39H1/H3K9me3 attenuates sulforaphane-induced apoptotic signaling in PC3 prostate cancer cells.  |  Watson, GW., et al. 2014. Oncogenesis. 3: e131. PMID: 25486523
10. HMGB1-mediated chromatin remodeling attenuates Il24 gene expression for the protection from allergic contact dermatitis.  |  Senda, N., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33443188

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Trimethyl Histone H3 Antibody (6F12-H4) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Trimethyl Histone H3 Antibody (6F12-H4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Trimethyl Histone H3 Antibody (6F12-H4) Specifications

Alternate Product Name: Trimethyl Histone H3

Product Clone Name: 6F12-H4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 6-16

Epitope Target: Lys 9 trimethylated Histone H3

Epitope Species: human

Product Reactivity/Detection: Lys 9 trimethylated Histone H3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Trimethyl Histone H3 Antibody (6F12-H4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent