TRIM69 CRISPR/Cas9 KO Plasmid (h): sc-404906
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRIM69 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TRIM69 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRIM69 CRISPR/Cas9 KO Plasmid (h) | sc-404906 | 20 µg | $397.00 |
Overview
TRIM69 encodes a tripartite motif (TRIM) family E3 ubiquitin ligase characterized by RING, B-box, and coiled-coil domains that mediate protein–protein interactions and ubiquitin-dependent signaling. TRIM69 has been implicated in innate immune regulation, including modulation of interferon-stimulated pathways and antiviral restriction mechanisms, through control of substrate stability and downstream transcriptional responses. By influencing ubiquitination and proteostasis, TRIM69 can shape cellular stress responses and signaling networks that intersect with inflammatory programs. Altered TRIM family activity is broadly relevant to immune dysregulation and oncogenic signaling, making TRIM69 a useful target for mechanistic studies in these contexts.
TRIM69 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TRIM69 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TRIM69 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TRIM69 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TRIM69 protein expression.
This CRISPR knockout system enables efficient generation of TRIM69-deficient cell models for investigation of TRIM69 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TRIM69 exon(s) critical for TRIM69 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TRIM69 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TRIM69 CRISPR/Cas9 KO Plasmid (h) and TRIM69 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TRIM69 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TRIM69 HDR Plasmid (h) and TRIM69 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TRIM69 homology arms to support homology-directed repair at defined TRIM69 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.