TRIM66 Antibody (A-9): sc-515177

TRIM66 Antibody (A-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects TRIM66 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TRIM66 Antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TRIM66, also known as tripartite motif-containing protein 66, TIF1D, C11orf29, or KIAA0298, is a 1,216 amino acid protein that plays a crucial role in cellular processes such as transcription regulation and chromatin remodeling. TRIM66 is characterized by a unique structure, which includes two B box-type zinc fingers, one bromo domain, and one PHD-type zinc finger, all contributing to function as a transcriptional repressor. TRIM66 is predominantly localized in the nucleus and is strongly expressed in the testis, thymus, and kidney, with moderate expression in the prostate and ovary. TRIM66′s ability to form individual foci in the centrometric chromocenter and surrounding nucleoplasm highlights importance in maintaining genomic stability and regulating gene expression. TRIM66 exists in two alternatively spliced isoforms, and the gene encoding TRIM66 is located on human chromosome 11p15.4, underscoring significance in developmental processes and potential implications in oncogenesis.

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TRIM66 Antibody (A-9) References:

1. TIF1delta, a novel HP1-interacting member of the transcriptional intermediary factor 1 (TIF1) family expressed by elongating spermatids.  |  Khetchoumian, K., et al. 2004. J Biol Chem. 279: 48329-41. PMID: 15322135
2. Human chromosome 11 DNA sequence and analysis including novel gene identification.  |  Taylor, TD., et al. 2006. Nature. 440: 497-500. PMID: 16554811
3. A large new subset of TRIM genes highly diversified by duplication and positive selection in teleost fish.  |  van der Aa, LM., et al. 2009. BMC Biol. 7: 7. PMID: 19196451
4. TRIM proteins: another class of viral victims.  |  Munir, M. 2010. Sci Signal. 3: jc2. PMID: 20407122
5. SUMO E3 ligase activity of TRIM proteins.  |  Chu, Y. and Yang, X. 2011. Oncogene. 30: 1108-16. PMID: 20972456
6. Tripartite-motif proteins and innate immune regulation.  |  McNab, FW., et al. 2011. Curr Opin Immunol. 23: 46-56. PMID: 21131187
7. Functional interactions between ubiquitin E2 enzymes and TRIM proteins.  |  Napolitano, LM., et al. 2011. Biochem J. 434: 309-19. PMID: 21143188
8. TRIM66 hastens the malignant progression of non-small cell lung cancer via modulating MMP9-mediated TGF-β/SMAD pathway.  |  Chen, W., et al. 2022. Cytokine. 153: 155831. PMID: 35301175
9. A TRIM66/DAX1/Dux axis suppresses the totipotent 2-cell-like state in murine embryonic stem cells.  |  Zuo, F., et al. 2022. Cell Stem Cell. 29: 948-961.e6. PMID: 35659877
10. TRIM66 Promotes Malignant Progression of Non-Small-Cell Lung Cancer Cells via Targeting MMP9.  |  Xu, Y., et al. 2022. Comput Math Methods Med. 2022: 6058720. PMID: 35912155
11. Trim66's paternal deficiency causes intrauterine overgrowth.  |  Mielnicka, M., et al. 2024. Life Sci Alliance. 7: PMID: 38719749
12. Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro.  |  Nagase, T., et al. 1997. DNA Res. 4: 141-50. PMID: 9205841

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TRIM66 Antibody (A-9) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TRIM66 Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TRIM66 Antibody (A-9) Specifications

Alternate Product Name: TRIM66

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1006-1026

Epitope Target: TRIM66

Epitope Species: human

Product Reactivity/Detection: TRIM66

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TRIM66 Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin