TRIM44 CRISPR/Cas9 KO Plasmid (h): sc-412330
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRIM44 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TRIM44 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRIM44 CRISPR/Cas9 KO Plasmid (h) | sc-412330 | 20 µg | $397.00 |
Overview
TRIM44 (tripartite motif containing 44) encodes a TRIM family protein implicated in ubiquitin-dependent regulation of protein stability and signaling output. Although atypical in domain composition compared with many TRIM E3 ligases, TRIM44 has been linked to modulation of proteostasis networks and stress-responsive signaling, influencing processes such as cell growth, survival, and motility. Dysregulated TRIM44 expression has been reported across multiple tumor types and is frequently associated with enhanced invasive phenotypes and altered pathway activity, including PI3K/AKT and NF-κB-associated signaling. These properties make TRIM44 a useful target for mechanistic studies of ubiquitin-linked regulation, pathway crosstalk, and context-dependent oncogenic signaling.
TRIM44 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TRIM44 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TRIM44 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TRIM44 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TRIM44 protein expression.
This CRISPR knockout system enables efficient generation of TRIM44-deficient cell models for investigation of TRIM44 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TRIM44 exon(s) critical for TRIM44 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TRIM44 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TRIM44 CRISPR/Cas9 KO Plasmid (h) and TRIM44 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TRIM44 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TRIM44 HDR Plasmid (h) and TRIM44 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TRIM44 homology arms to support homology-directed repair at defined TRIM44 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.