TRIM41 CRISPR/Cas9 KO Plasmid (h): sc-413871
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRIM41 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TRIM41 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRIM41 CRISPR/Cas9 KO Plasmid (h) | sc-413871 | 20 µg | $397.00 |
Overview
TRIM41 encodes a tripartite motif-containing (TRIM) protein with RING finger E3 ubiquitin ligase activity that contributes to ubiquitin-dependent proteostasis and regulation of signaling complexes. As part of TRIM family networks, TRIM41 is implicated in controlling protein stability and turnover, influencing pathways linked to innate immune regulation, stress responses, and maintenance of cellular homeostasis. Altered ubiquitination dynamics involving TRIM proteins have been associated with dysregulated inflammatory signaling and oncogenic phenotypes, making TRIM41 a useful target for dissecting ubiquitin pathway contributions to disease-relevant cell states. Functional interrogation of TRIM41 supports studies of substrate recognition, post-translational modification crosstalk, and pathway-level effects on transcriptional and proteomic outputs.
TRIM41 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TRIM41 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TRIM41 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TRIM41 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TRIM41 protein expression.
This CRISPR knockout system enables efficient generation of TRIM41-deficient cell models for investigation of TRIM41 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TRIM41 exon(s) critical for TRIM41 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TRIM41 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TRIM41 CRISPR/Cas9 KO Plasmid (h) and TRIM41 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TRIM41 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TRIM41 HDR Plasmid (h) and TRIM41 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TRIM41 homology arms to support homology-directed repair at defined TRIM41 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.