TRIM16 Double Nickase Plasmid (h): sc-409792-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRIM16 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TRIM16 Double Nickase Plasmid (h) and TRIM16 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TRIM16. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TRIM16 Antibody (B-6): sc-398851
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRIM16 Double Nickase Plasmid (h) | sc-409792-NIC | 20 µg | $410.00 | |||
TRIM16 Double Nickase Plasmid (h2) | sc-409792-NIC-2 | 20 µg | $410.00 |
TRIM16 encodes a tripartite motif (TRIM) family protein that functions as an E3 ubiquitin ligase and scaffolding factor coordinating protein turnover, cytoskeletal dynamics, and stress-responsive signaling. TRIM16 has been linked to regulation of intermediate filament organization, proteostasis, and modulation of transcriptional programs that influence cell differentiation and motility. Through ubiquitin-dependent mechanisms and protein–protein interactions, TRIM16 can impact pathways governing innate immune responses, oxidative stress adaptation, and growth control. Altered TRIM16 expression or activity has been associated with tumor biology and inflammatory phenotypes, making it a useful target for mechanistic studies of signaling networks and cellular homeostasis.
TRIM16 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TRIM16 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TRIM16. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TRIM16 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TRIM16-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.