TRF2 CRISPR/Cas9 KO Plasmid (m): sc-423340
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRF2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TRF2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TRF2 Antibody (9F10): sc-47693
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRF2 CRISPR/Cas9 KO Plasmid (m) | sc-423340 | 20 µg | $397.00 |
Overview
Mouse Terf2 encodes telomeric repeat-binding factor 2 (TRF2), a core component of the shelterin complex that binds double-stranded telomeric DNA and promotes t-loop formation to protect chromosome ends. TRF2 suppresses inappropriate activation of the ATM-dependent DNA damage response and limits end-to-end chromosome fusions by preventing non-homologous end joining at telomeres, thereby supporting genome stability and faithful cell-cycle progression. Disruption of TRF2 function drives telomere uncapping, replication stress, and chromosomal aberrations, processes relevant to aging biology, stem cell attrition, and tumorigenesis research. Terf2 is therefore widely used as a handle to study telomere maintenance pathways and crosstalk between telomeres, DNA repair, and checkpoint signaling.
TRF2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Terf2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Terf2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Terf2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TRF2 protein expression.
This CRISPR knockout system enables efficient generation of Terf2-deficient cell models for investigation of TRF2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Terf2 exon(s) critical for TRF2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Terf2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TRF2 CRISPR/Cas9 KO Plasmid (m) and TRF2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Terf2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TRF2 HDR Plasmid (m) and TRF2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Terf2 homology arms to support homology-directed repair at defined Terf2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.