TREM-2 Double Nickase Plasmid (h): sc-401585-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TREM-2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TREM-2 Double Nickase Plasmid (h) and TREM-2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TREM2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TREM-2 Antibody (B-3): sc-373828
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TREM-2 Double Nickase Plasmid (h) | sc-401585-NIC | 20 µg | $410.00 | |||
TREM-2 Double Nickase Plasmid (h2) | sc-401585-NIC-2 | 20 µg | $410.00 |
TREM2 encodes the triggering receptor expressed on myeloid cells 2 (TREM-2), an immunoreceptor primarily expressed by microglia and other tissue macrophages that shapes innate immune surveillance and homeostatic responses. Through adaptor coupling to TYROBP/DAP12, TREM-2 signaling engages SYK-dependent pathways to regulate phagocytosis, lipid sensing, cell survival, and modulation of inflammatory cytokine programs. TREM-2 influences microglial activation states, debris clearance, and responses to neurodegenerative pathology, and genetic or functional perturbation has been linked to altered risk and progression in disorders such as Alzheimer’s disease and other dementias. In peripheral myeloid compartments, TREM-2 also contributes to macrophage phenotypes relevant to chronic inflammation and tissue remodeling.
TREM-2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TREM2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TREM2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TREM2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TREM2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.