TRBP2 Antibody (D-5) recommended for detection of TRBP2 of human origin by WB, IP, IF and ELISA

TRBP2 Antibody (D-5): sc-514124

TRBP2 Antibody (D-5) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-TRBP2 Antibody (D-5) is a mouse monoclonal IgG2b (kappa light chain) TRBP2 antibody provided at 200 µg/ml
    • raised against amino acids 68-124 mapping near the N-terminus of TRBP2 of human origin
    • TRBP2 Antibody (D-5) is recommended for detection of TRBP2 of human origin by WB, IP, IF and ELISA
    • Anti-TRBP2 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TRBP2 (D-5): sc-514124.
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What is the recommended starting dilution for Western blot for sc-514124: TRBP2 (D-5) monoclonal antibody?

Asked by: jenni
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from a good antibody for western analsysis This antibody detects a good signal (in the form of triplet or quadruplet) in the expect M.W. range. A similar band pattern is also observed when using a polyclonal antibody purchased from another supplier.
Date published: 2019-12-16
Rated 5 out of 5 by from Works for Western blot Used human THP-1 cell line +/- TRBP2 CRISPR deletion. 1x10e6 cells lysed in 100uL of RIPA buffer, no stimulation. WB Protocol: Transferred onto PVDF membrane Blocked 1hr with 5% Skim Milk in TBST Antibody over night at 4oC, 1:1,000 (5% Skim Milk in TBST) Detected with anti-mouse-HRP (1:10,000)
Date published: 2018-11-21
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of TRBP2 expression in MCF7, Hep G2, HEK293T, HeLa and NTERA-2 cl.D1 whole cell lysates.
Date published: 2017-02-06
Rated 5 out of 5 by from Great Western blot data of TRBP2 expression Great Western blot data of TRBP2 expression in MCF7, Hep G2, HEK293T, HeLa and NTERA-2 cl.D1 whole cell lysates. -SCBT QC
Date published: 2014-09-28
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