TRB-2 Antibody (F-5): sc-376776

TRB-2 Antibody (F-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects TRB-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TRB-2 (F-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TRB-2, also known as Tribbles homolog 2, TRIB2, C5FW, or GS3955, is a cytoplasmic pro-apoptotic protein that plays a crucial role in regulating apoptosis and cell signaling pathways. TRB-2 belongs to the Tribbles subfamily of the Serine/Threonine protein kinase family, characterized by a protein kinase-like domain lacking the active site lysine, indicating no traditional kinase activity. TRB-2 interacts with mitogen-activated protein kinase kinases (MAPKKs), influencing MAP kinase signaling pathway, which is vital for cellular responses to growth factors and stress signals. TRB-2 expression is predominantly found in peripheral blood leukocytes, highlighting a potential role in immune responses and hematopoiesis. Understanding TRB-2 function and regulation is essential for elucidating involvement in various diseases, including cancer and metabolic disorders, making anti-TRB-2 antibody (F-5) a valuable tool for researchers studying these critical biological processes.

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TRB-2 Antibody (F-5) References:

1. SINK is a p65-interacting negative regulator of NF-kappaB-dependent transcription.  |  Wu, M., et al. 2003. J Biol Chem. 278: 27072-9. PMID: 12736262
2. Human tribbles, a protein family controlling mitogen-activated protein kinase cascades.  |  Kiss-Toth, E., et al. 2004. J Biol Chem. 279: 42703-8. PMID: 15299019
3. Identification of tribbles homolog 2 as an autoantigen in autoimmune uveitis by phage display.  |  Zhang, Y., et al. 2005. Mol Immunol. 42: 1275-81. PMID: 15950723
4. Tribbles homolog 2 inactivates C/EBPalpha and causes acute myelogenous leukemia.  |  Keeshan, K., et al. 2006. Cancer Cell. 10: 401-11. PMID: 17097562
5. Survival factor withdrawal-induced apoptosis of TF-1 cells involves a TRB2-Mcl-1 axis-dependent pathway.  |  Lin, KR., et al. 2007. J Biol Chem. 282: 21962-72. PMID: 17545167
6. TRB2, a mouse Tribbles ortholog, suppresses adipocyte differentiation by inhibiting AKT and C/EBPbeta.  |  Naiki, T., et al. 2007. J Biol Chem. 282: 24075-82. PMID: 17576771
7. Tribbles homolog 2 (Trib2) and HoxA9 cooperate to accelerate acute myelogenous leukemia.  |  Keeshan, K., et al. 2008. Blood Cells Mol Dis. 40: 119-21. PMID: 17988908
8. Upregulation of TRB2 induced by miR-98 in the early lesions of large artery of type-2 diabetic rat.  |  Xie, S., et al. 2012. Mol Cell Biochem. 361: 305-14. PMID: 22012613
9. Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad ubiquitination regulatory factor 1 (Smurf1) promote tribbles homolog 2 (TRIB2) stability and carcinogenic property in liver cancer.  |  Wang, J., et al. 2013. J Biol Chem. 288: 33667-33681. PMID: 24089522
10. Knockdown of circ_0084043 suppresses the development of human melanoma cells through miR-429/tribbles homolog 2 axis and Wnt/β-catenin pathway.  |  Chen, Z., et al. 2020. Life Sci. 243: 117323. PMID: 31954160
11. Tribbles homolog 2 (Trib2), a pseudo serine/threonine kinase in tumorigenesis and stem cell fate decisions.  |  Fang, Y., et al. 2021. Cell Commun Signal. 19: 41. PMID: 33794905
12. Characterization of a phosphoprotein whose mRNA is regulated by the mitogenic pathways in dog thyroid cells.  |  Wilkin, F., et al. 1997. Eur J Biochem. 248: 660-8. PMID: 9342215

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TRB-2 Antibody (F-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TRB-2 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TRB-2 Antibody (F-5) Specifications

Alternate Product Name: TRB-2

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 11-63

Epitope Target: TRB-2

Epitope Species: human

Product Reactivity/Detection: TRB-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TRB-2 Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin