TRAP220 Antibody (H-7): sc-74475

TRAP220 Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects TRAP220 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TRAP220 (H-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TRAP220 plays a crucial role in regulating transcription within mammalian cells, functioning as a key component of the thyroid hormone receptor-associated protein (TRAP) complex. This complex mediates interaction between transcriptional activators and transcriptional machinery, facilitating hormone-induced transcriptional activation. TRAP220, along with other proteins in the TRAP complex, contains a nuclear receptor recognition motif enabling ligand-dependent binding to nuclear receptors, which is vital for proper response to hormonal signals. TRAP220 and TRAP100 are predominantly expressed in tissues such as skeletal muscle, heart, and placenta, highlighting their importance in developmental and physiological processes. TRAP220′s interaction with other components of the TRAP complex, including TRAP95, TRAP150, and TRAP230, underscores a role in enhancing transcriptional activation, making TRAP220 a significant target for studies related to gene expression regulation and hormonal signaling pathways.

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TRAP220 Antibody (H-7) References:

1. A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation.  |  Gu, W., et al. 1999. Mol Cell. 3: 97-108. PMID: 10024883
2. Competition between thyroid hormone receptor-associated protein (TRAP) 220 and transcriptional intermediary factor (TIF) 2 for binding to nuclear receptors. Implications for the recruitment of TRAP and p160 coactivator complexes.  |  Treuter, E., et al. 1999. J Biol Chem. 274: 6667-77. PMID: 10037764
3. Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators.  |  Ito, M., et al. 1999. Mol Cell. 3: 361-70. PMID: 10198638
4. Identification of mouse TRAP100: a transcriptional coregulatory factor for thyroid hormone and vitamin D receptors.  |  Zhang, J. and Fondell, JD. 1999. Mol Endocrinol. 13: 1130-40. PMID: 10406464
5. The structure of the nuclear hormone receptors.  |  Kumar, R. and Thompson, EB. 1999. Steroids. 64: 310-9. PMID: 10406480
6. Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated adipogenesis.  |  Ge, K., et al. 2002. Nature. 417: 563-7. PMID: 12037571
7. An extended LXXLL motif sequence determines the nuclear receptor binding specificity of TRAP220.  |  Coulthard, VH., et al. 2003. J Biol Chem. 278: 10942-51. PMID: 12556447
8. Structural and functional organization of TRAP220, the TRAP/mediator subunit that is targeted by nuclear receptors.  |  Malik, S., et al. 2004. Mol Cell Biol. 24: 8244-54. PMID: 15340084
9. Activation of TRAP/mediator subunit TRAP220/Med1 is regulated by mitogen-activated protein kinase-dependent phosphorylation.  |  Pandey, PK., et al. 2005. Mol Cell Biol. 25: 10695-710. PMID: 16314496
10. Clinical Significance of Peroxisome Proliferator-Activated Receptor γ and TRAP220 in Patients with Operable Colorectal Cancer.  |  Kwon, KA., et al. 2016. Cancer Res Treat. 48: 198-207. PMID: 26130665
11. The TRAP220 component of a thyroid hormone receptor- associated protein (TRAP) coactivator complex interacts directly with nuclear receptors in a ligand-dependent fashion.  |  Yuan, CX., et al. 1998. Proc Natl Acad Sci U S A. 95: 7939-44. PMID: 9653119
12. Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways.  |  Jiang, YW., et al. 1998. Proc Natl Acad Sci U S A. 95: 8538-43. PMID: 9671713

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TRAP220 Antibody (H-7) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-TRAP220 Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TRAP220 Antibody (H-7) Specifications

Alternate Product Name: Mediator Complex Subunit 1 (MED1)

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 502-756

Epitope Target: TRAP220

Epitope Species: mouse

Product Reactivity/Detection: TRAP220

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for TRAP220 Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent