TRAP220 Antibody (B-4): sc-514935

TRAP220 Antibody (B-4) is a mouse monoclonal IgG2a antibody that detects TRAP220 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TRAP220 plays a crucial role in transcription regulation by serving as a key component of the thyroid hormone receptor-associated protein (TRAP) complex, which mediates interaction between transcriptional activators and initiation factors. TRAP220 is primarily located in the nucleus, where TRAP220 facilitates hormone-induced transcriptional activation by binding to nuclear receptors, including thyroid hormone receptors and vitamin D receptors. Nuclear localization of TRAP220 allows precise regulation of gene expression in response to hormonal signals, impacting various physiological processes such as metabolism, development, and cellular differentiation. TRAP220 is widely expressed in tissues such as skeletal muscle, heart, and placenta, highlighting TRAP220′s importance in both developmental and metabolic pathways. Through interactions with other components of the TRAP complex, TRAP220 is integral to forming functional transcriptional machinery, making TRAP220 a significant target for studies related to gene regulation and endocrine signaling.

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TRAP220 Antibody (B-4) References:

1. Involvement of the TRAP220 component of the TRAP/SMCC coactivator complex in embryonic development and thyroid hormone action.  |  Ito, M., et al. 2000. Mol Cell. 5: 683-93. PMID: 10882104
2. Specific structural motifs determine TRAP220 interactions with nuclear hormone receptors.  |  Ren, Y., et al. 2000. Mol Cell Biol. 20: 5433-46. PMID: 10891484
3. TRAP220 is modulated by the antineoplastic agent 6-Mercaptopurine, and mediates the activation of the NR4A subgroup of nuclear receptors.  |  Wansa, KD. and Muscat, GE. 2005. J Mol Endocrinol. 34: 835-48. PMID: 15956351
4. MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription.  |  Zhang, X., et al. 2005. Mol Cell. 19: 89-100. PMID: 15989967
5. Thyroid hormone-induced juxtaposition of regulatory elements/factors and chromatin remodeling of Crabp1 dependent on MED1/TRAP220.  |  Park, SW., et al. 2005. Mol Cell. 19: 643-53. PMID: 16137621
6. The role of transcriptional coactivator TRAP220 in myelomonocytic differentiation.  |  Urahama, N., et al. 2005. Genes Cells. 10: 1127-37. PMID: 16324150
7. Regulation of Aurora-A kinase gene expression via GABP recruitment of TRAP220/MED1.  |  Udayakumar, TS., et al. 2006. J Biol Chem. 281: 14691-9. PMID: 16574658
8. The Mediator subunit MED1/TRAP220 is required for optimal glucocorticoid receptor-mediated transcription activation.  |  Chen, W. and Roeder, RG. 2007. Nucleic Acids Res. 35: 6161-9. PMID: 17827210
9. A different TRAP220 expression in distinct histologic subtypes of lung adenocarcinoma and the prognostic significance.  |  Yun, J., et al. 2011. Lung Cancer. 71: 312-8. PMID: 20650541
10. Checkpoint-dependent phosphorylation of Med1/TRAP220 in response to DNA damage.  |  Kim, HJ. and Yun, J. 2017. Acta Biochim Biophys Sin (Shanghai). 49: 496-502. PMID: 28430840

The preferred TRAP220 antibody is TRAP220 Antibody (H-7): sc-74475.