TRAP150 Double Nickase Plasmid (m): sc-432980-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRAP150 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TRAP150 Double Nickase Plasmid (m) and TRAP150 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Thrap3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TRAP150 Antibody (F-10): sc-133250
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRAP150 Double Nickase Plasmid (m) | sc-432980-NIC | 20 µg | $410.00 | |||
TRAP150 Double Nickase Plasmid (m2) | sc-432980-NIC-2 | 20 µg | $410.00 |
Mouse Thrap3 encodes TRAP150, a nuclear RNA-binding and transcription-associated factor implicated in coupling transcription with pre-mRNA splicing and 3′ end processing. TRAP150 participates in mRNP maturation and interacts with components of the spliceosome and transcriptional machinery, influencing alternative splicing decisions and gene expression programs. These functions connect Thrap3 to regulation of cell-cycle progression and stress-responsive transcriptional networks that are frequently perturbed in cancer biology. Altered splicing and transcriptional coordination associated with TRAP150 activity makes this gene relevant for studying mechanisms underlying oncogenic gene expression and RNA processing defects.
TRAP150 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Thrap3 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Thrap3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Thrap3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Thrap3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.