Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TRAP-1 CRISPR/Cas9 KO Plasmid (h): sc-402963
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRAP-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TRAP-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TRAP-1 Antibody (C-8): sc-13134
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRAP-1 CRISPR/Cas9 KO Plasmid (h) | sc-402963 | 20 µg | $397.00 |
Overview
TGFBRAP1 encodes transforming growth factor beta receptor–associated protein 1 (TRAP-1), a cytoplasmic adaptor implicated in modulating TGF-β superfamily signaling. TRAP-1 has been reported to associate with components of the TGF-β receptor complex and influence downstream SMAD-dependent transcriptional responses that regulate proliferation, differentiation, extracellular matrix remodeling, and immune-related gene programs. Through its role in shaping pathway output and receptor-proximal events, TGFBRAP1 is studied in the context of signaling crosstalk and cellular state transitions. Altered TGF-β pathway regulation is broadly relevant to fibrosis, cancer biology, and inflammatory disease mechanisms, making TRAP-1 a useful target for mechanistic pathway studies.
TRAP-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TGFBRAP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TGFBRAP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TGFBRAP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TRAP-1 protein expression.
This CRISPR knockout system enables efficient generation of TGFBRAP1-deficient cell models for investigation of TRAP-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TGFBRAP1 exon(s) critical for TRAP-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TGFBRAP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TRAP-1 CRISPR/Cas9 KO Plasmid (h) and TRAP-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TGFBRAP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TRAP-1 HDR Plasmid (h) and TRAP-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TGFBRAP1 homology arms to support homology-directed repair at defined TGFBRAP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.