Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
transgelin Double Nickase Plasmid (h): sc-401343-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- transgelin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- transgelin Double Nickase Plasmid (h) and transgelin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TAGLN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: transgelin Antibody (6G6): sc-53932
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
transgelin Double Nickase Plasmid (h) | sc-401343-NIC | 20 µg | $410.00 | |||
transgelin Double Nickase Plasmid (h2) | sc-401343-NIC-2 | 20 µg | $410.00 |
TAGLN encodes transgelin, an actin-binding protein enriched in smooth muscle and myofibroblast-like cells that stabilizes filamentous actin and supports contractile architecture. Transgelin participates in cytoskeletal remodeling, cell shape regulation, and motility programs downstream of RhoA/ROCK and other actin dynamics pathways, influencing adhesion and extracellular matrix interactions. Altered TAGLN expression is frequently used as a marker of smooth muscle differentiation and phenotypic switching, and it has been associated with changes in tissue remodeling and tumor–stroma biology in multiple contexts. These features make TAGLN a practical target for dissecting how actin organization and contractility control cell migration, mechanotransduction, and lineage-associated gene programs.
transgelin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TAGLN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TAGLN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TAGLN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TAGLN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.