transgelin Antibody (6G6): sc-53932
- transgelin Antibody (6G6) is a mouse monoclonal IgG1 κ transgelin antibody, cited in 31 publications, provided at 200 µg/ml
- raised against full length transgelin of human origin
- transgelin Antibody (6G6) is recommended for detection of transgelin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-transgelin Antibody (6G6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for transgelin Antibody (6G6) for WB and IHC(P) applications. These reagents are now offered in bundles with transgelin Antibody (6G6) (see ordering information below).
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transgelin Antibody (6G6) is a mouse monoclonal IgG1 kappa light chain antibody that detects transgelin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-transgelin antibody (6G6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Transgelin, also known as SM22a, plays a crucial role in regulating smooth muscle contraction and maintaining cytoskeletal structural integrity. Transgelin is primarily located in smooth muscle cells, contributing to actin filament organization and influencing cellular shape and motility. Transgelin presence in smooth muscle cells is vital for proper vascular function and development, helping regulate cellular contractile state. The human transgelin gene (TAGLN) is situated on chromosome 11q23.3 and encodes a 201 amino acid protein containing nuclear factor-binding motifs, important for transcriptional regulation in smooth muscle. During embryonic development, transgelin is expressed in various muscle types, but expression becomes restricted to vascular and visceral smooth muscle cells in adulthood, with lower levels in cardiac tissue. Transgelin is downregulated in several transformed cell lines, suggesting decreased expression may serve as an early biomarker for cellular transformation. Transgelin interacts with actin, promoting actin fiber gelling shortly after binding, with a binding ratio of 1:6 actin monomers, highlighting transgelin′s significant role in cytoskeletal dynamics.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
transgelin Antibody (6G6) References:
- Purification, characterization, and partial sequence analysis of a new 25-kDa actin-binding protein from bovine aorta: a SM22 homolog. | Kobayashi, R., et al. 1994. Biochem Biophys Res Commun. 198: 1275-80. PMID: 8117285
- Purification and properties of transgelin: a transformation and shape change sensitive actin-gelling protein. | Shapland, C., et al. 1993. J Cell Biol. 121: 1065-73. PMID: 8501116
- Evidence for serum response factor-mediated regulatory networks governing SM22alpha transcription in smooth, skeletal, and cardiac muscle cells. | Li, L., et al. 1997. Dev Biol. 187: 311-21. PMID: 9242426
- Fibroblast transgelin and smooth muscle SM22alpha are the same protein, the expression of which is down-regulated in many cell lines. | Lawson, D., et al. 1997. Cell Motil Cytoskeleton. 38: 250-7. PMID: 9384215
- Expression and cytogenetic localization of the human SM22 gene (TAGLN). | Camoretti-Mercado, B., et al. 1998. Genomics. 49: 452-7. PMID: 9615232
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
transgelin Antibody (6G6) | sc-53932 | 200 µg/ml | $316.00 | |||
transgelin Antibody (6G6): m-IgG Fc BP-HRP Bundle | sc-528588 | 200 µg Ab; 10 µg BP | $354.00 | |||
transgelin Antibody (6G6): m-IgGκ BP-HRP Bundle | sc-521000 | 200 µg Ab, 40 µg BP | $354.00 | |||
transgelin Antibody (6G6): m-IgG1 BP-HRP Bundle | sc-543012 | 200 µg Ab; 20 µg BP | $354.00 | |||
transgelin Antibody (6G6) AC | sc-53932 AC | 500 µg/ml, 25% agarose | $416.00 | |||
transgelin Antibody (6G6) HRP | sc-53932 HRP | 200 µg/ml | $316.00 | |||
transgelin Antibody (6G6) FITC | sc-53932 FITC | 200 µg/ml | $330.00 | |||
transgelin Antibody (6G6) PE | sc-53932 PE | 200 µg/ml | $343.00 | |||
transgelin Antibody (6G6) Alexa Fluor® 488 | sc-53932 AF488 | 200 µg/ml | $357.00 | |||
transgelin Antibody (6G6) Alexa Fluor® 546 | sc-53932 AF546 | 200 µg/ml | $357.00 | |||
transgelin Antibody (6G6) Alexa Fluor® 594 | sc-53932 AF594 | 200 µg/ml | $357.00 | |||
transgelin Antibody (6G6) Alexa Fluor® 647 | sc-53932 AF647 | 200 µg/ml | $357.00 | |||
transgelin Antibody (6G6) Alexa Fluor® 680 | sc-53932 AF680 | 200 µg/ml | $357.00 | |||
transgelin Antibody (6G6) Alexa Fluor® 790 | sc-53932 AF790 | 200 µg/ml | $357.00 |