TRα1/β1 Antibody (C4): sc-740
- TRα1/β1 Antibody (C4) is a mouse monoclonal IgG1 κ TRα1/β1 antibody, cited in 16 publications, provided at 200 µg/ml
- epitope mapping within the C-terminus of TRα1 of human origin
- TR alpha 1/ beta 1 Antibody (C4) is recommended for detection of TRα1 and TRβ1 of human origin by WB, IP and IF
- Anti-TR alpha 1/ beta 1 Antibody (C4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-740 X, 200 µg/0.1 ml)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TRα1/β1 Antibody (C4). This work is in progress.
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TRα1/β1 Antibody (C4) is a mouse monoclonal IgG1 kappa light chain antibody that detects the TR alpha 1/beta 1 protein of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). TRα1/β1 (C4) monoclonal antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Thyroid hormone nuclear receptors (TRs) are crucial ligand-dependent transcription factors that play a significant role in regulating metabolic and developmental processes. TRα1 can localize to both the nucleus and the cytoplasm, and is the only TR imported into the mitochondrial matrix, highlighting its importance in cellular metabolism and energy regulation. This localization allows TRα1 to influence mitochondrial gene expression and function, thereby impacting cellular respiration and energy production. TRs bind to thyroid hormone response elements (TREs) with varying affinities, which are influenced by the presence of 3,5,3′-triiodo-L-thyronine (T3). TR transcriptional activity is further modulated through heterodimerization with retinoid X receptors (RXRα, RXRβ, and RXRγ), which enhances regulatory capabilities. Understanding TRα1 function and localization is essential for elucidating its role in thyroid hormone signaling and implications in metabolic disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TRα1/β1 Antibody (C4) References:
- Normal timing of oligodendrocyte development depends on thyroid hormone receptor alpha 1 (TRalpha1). | Billon, N., et al. 2002. EMBO J. 21: 6452-60. PMID: 12456652
- The orientation and spacing of core DNA-binding motifs dictate selective transcriptional responses to three nuclear receptors. | Näär, AM., et al. 1991. Cell. 65: 1267-79. PMID: 1648451
- Thyroid hormone receptor TRbeta1 mediates Akt activation by T3 in pancreatic beta cells. | Verga Falzacappa, C., et al. 2007. J Mol Endocrinol. 38: 221-33. PMID: 17293442
- Physiological consequences of the TRalpha1 aporeceptor state. | Mittag, J., et al. 2010. Heart Fail Rev. 15: 111-5. PMID: 19009345
- [Effects of 2,2',4,4'-tetrabrominated diphenyl ethers on the TRalpha1 and TRbeta1 expression in liver in C57BL/6 mice]. | Liu, Z., et al. 2010. Wei Sheng Yan Jiu. 39: 399-403. PMID: 20726222
- The two major isoforms of thyroid hormone receptor, TRα1 and TRβ1, preferentially partner with distinct panels of auxiliary proteins. | Hahm, JB., et al. 2014. Mol Cell Endocrinol. 383: 80-95. PMID: 24325866
- Impact of the thyroid hormone T3 and its nuclear receptor TRα1 on colon cancer stem cell phenotypes and response to chemotherapies. | Giolito, MV., et al. 2024. Cell Death Dis. 15: 306. PMID: 38693105
- Phosphorylation enhances the target gene sequence-dependent dimerization of thyroid hormone receptor with retinoid X receptor. | Bhat, MK., et al. 1994. Proc Natl Acad Sci U S A. 91: 7927-31. PMID: 8058736
- Hetero- and homodimeric receptors in thyroid hormone and vitamin A action. | Zhang, XK. and Pfahl, M. 1993. Receptor. 3: 183-91. PMID: 8167569
- Phosphorylation selectively increases triiodothyronine receptor homodimer binding to DNA. | Sugawara, A., et al. 1994. J Biol Chem. 269: 433-7. PMID: 8276832
- Thyroid hormone receptors: multiple forms, multiple possibilities. | Lazar, MA. 1993. Endocr Rev. 14: 184-93. PMID: 8325251
- Interaction of human beta 1 thyroid hormone receptor and its mutants with DNA and retinoid X receptor beta. T3 response element-dependent dominant negative potency. | Meier, CA., et al. 1993. J Clin Invest. 92: 1986-93. PMID: 8408652
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRα1/β1 Antibody (C4) | sc-740 | 200 µg/ml | $316.00 | |||
TRα1/β1 Antibody (C4) X | sc-740 X | 200 µg/0.1 ml | $316.00 | |||
TRα1/β1 Antibody (C4) AC | sc-740 AC | 500 µg/ml, 25% agarose | $416.00 | |||
TRα1/β1 Antibody (C4) HRP | sc-740 HRP | 200 µg/ml | $316.00 | |||
TRα1/β1 Antibody (C4) FITC | sc-740 FITC | 200 µg/ml | $330.00 | |||
TRα1/β1 Antibody (C4) PE | sc-740 PE | 200 µg/ml | $343.00 | |||
TRα1/β1 Antibody (C4) Alexa Fluor® 488 | sc-740 AF488 | 200 µg/ml | $357.00 | |||
TRα1/β1 Antibody (C4) Alexa Fluor® 546 | sc-740 AF546 | 200 µg/ml | $357.00 | |||
TRα1/β1 Antibody (C4) Alexa Fluor® 594 | sc-740 AF594 | 200 µg/ml | $357.00 | |||
TRα1/β1 Antibody (C4) Alexa Fluor® 647 | sc-740 AF647 | 200 µg/ml | $357.00 | |||
TRα1/β1 Antibody (C4) Alexa Fluor® 680 | sc-740 AF680 | 200 µg/ml | $357.00 | |||
TRα1/β1 Antibody (C4) Alexa Fluor® 790 | sc-740 AF790 | 200 µg/ml | $357.00 |