TRα1/α2 Antibody (3C185): sc-73213

TRα1/α2 Antibody (3C185) is a mouse monoclonal IgG1 antibody that detects TRα1/α2 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. TRα1/α2 plays a crucial role as a ligand-dependent transcription factor that regulates numerous metabolic and developmental processes, making TRα1/α2 essential for maintaining homeostasis within the body. TRα1 is unique as TRα1 can localize both in the nucleus and cytoplasm, and TRα1 is the only thyroid hormone receptor imported into the mitochondrial matrix, highlighting TRα1′s diverse functional roles. In contrast, TRα2, a C-terminal variant of TRα1, does not bind thyroid hormones and acts as a dominant negative antagonist of thyroid hormone signaling, thereby influencing the overall regulatory mechanisms of thyroid hormone action. The ability of TRs to bind to thyroid hormone response elements with varying affinities, influenced by 3,5,3′-triiodo-L-thyronine (T3), further underscores the complexity of their regulatory functions, which are also modulated through heterodimerization with retinoid X receptors. Anti-TRα1/α2 antibody (3C185) is an invaluable tool for researchers aiming to explore the intricate roles of thyroid hormone receptors in cellular processes and their implications in various physiological and pathological conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TRα1/α2 Antibody (3C185) References:

1. Elevated expression of thyroid hormone receptor alpha 2 (c-erb A- alpha 2) in nasopharyngeal carcinoma.  |  Lee, JW., et al. 2000. Br J Cancer. 83: 1674-80. PMID: 11104565
2. Deletion of the thyroid hormone receptor alpha 1 prevents the structural alterations of the cerebellum induced by hypothyroidism.  |  Morte, B., et al. 2002. Proc Natl Acad Sci U S A. 99: 3985-9. PMID: 11891331
3. Thyroid hormone receptor alpha 1 (c-erb A alpha 1) suppressed transforming phenotype of nasopharyngeal carcinoma cell line.  |  Lee, JW., et al. 2002. Cancer Lett. 184: 149-56. PMID: 12127686
4. The unique C-termini of the thyroid hormone receptor variant, c-erbA alpha 2, and thyroid hormone receptor alpha 1 mediate different DNA-binding and heterodimerization properties.  |  Katz, D., et al. 1992. Mol Endocrinol. 6: 805-14. PMID: 1318505
5. Time-dependent changes in the expression of thyroid hormone receptor alpha 1 in the myocardium after acute myocardial infarction: possible implications in cardiac remodelling.  |  Pantos, C., et al. 2007. Eur J Endocrinol. 156: 415-24. PMID: 17389455
6. Direct and indirect consequences on gene expression of a thyroid hormone receptor alpha 1 mutation restricted to Sertoli cells.  |  Chatonnet, F., et al. 2014. Mol Reprod Dev. 81: 1159-66. PMID: 25487743
7. The Antiarrhythmic Drug, Dronedarone, Demonstrates Cytotoxic Effects in Breast Cancer Independent of Thyroid Hormone Receptor Alpha 1 (THRα1) Antagonism.  |  Elliott, MJ., et al. 2018. Sci Rep. 8: 16562. PMID: 30410118
8. Regulation of neuronal thyroid hormone receptor alpha 1 mRNA by hydrocortisone, thyroid hormone and retinoic acid.  |  Satyanarayana, M., et al. 1994. Dev Neurosci. 16: 255-9. PMID: 7768204
9. Nonbiased identification of DNA sequences that bind thyroid hormone receptor alpha 1 with high affinity.  |  Katz, RW. and Koenig, RJ. 1993. J Biol Chem. 268: 19392-7. PMID: 8366086
10. The D domain of the thyroid hormone receptor alpha 1 specifies positive and negative transcriptional regulation functions.  |  Lee, Y. and Mahdavi, V. 1993. J Biol Chem. 268: 2021-8. PMID: 8420976

The preferred TRα1/β1 antibody is TRα1/β1 Antibody (C4): sc-740.