Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TRAF6 CRISPR/Cas9 KO Plasmid (m): sc-423497
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRAF6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TRAF6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TRAF6 Antibody (D-10): sc-8409
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRAF6 CRISPR/Cas9 KO Plasmid (m) | sc-423497 | 20 µg | $397.00 |
Overview
Mouse Traf6 encodes TRAF6, an E3 ubiquitin ligase and adaptor that integrates signaling downstream of TNF receptor superfamily members and Toll-like/IL-1 receptors to control innate and adaptive immune responses. TRAF6 catalyzes K63-linked ubiquitination events that promote assembly of TAK1 and IKK complexes, driving NF-κB and MAPK signaling and transcriptional programs regulating inflammation, cell survival, and differentiation. It is also central to RANK/RANKL signaling in osteoclastogenesis and contributes to autophagy regulation through ubiquitin-dependent scaffolding. Dysregulated TRAF6 signaling has been implicated in inflammatory pathology, bone remodeling defects, and oncogenic signaling contexts where NF-κB/MAPK activation and ubiquitin pathway rewiring are prominent.
TRAF6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Traf6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Traf6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Traf6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TRAF6 protein expression.
This CRISPR knockout system enables efficient generation of Traf6-deficient cell models for investigation of TRAF6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Traf6 exon(s) critical for TRAF6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Traf6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TRAF6 CRISPR/Cas9 KO Plasmid (m) and TRAF6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Traf6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TRAF6 HDR Plasmid (m) and TRAF6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Traf6 homology arms to support homology-directed repair at defined Traf6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.