TRAF5 Antibody (E-4): sc-74502

TRAF5 Antibody (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects TRAF5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TRAF5 Antibody (E-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TRAF5, a member of the TNF receptor-associated factor (TRAF) protein family, plays a crucial role in mediating signal transduction from members of the TNF receptor superfamily, which is essential for various cellular processes, including immune response and inflammation. TRAF5 is integral to the signaling complex associated with the CD40 cytoplasmic domain, facilitating tumor necrosis factor-induced NF-kappa B activation and providing protection against apoptosis. TRAF5 is involved in osteoclastogenesis, highlighting its importance in bone metabolism and immune regulation. TRAF5 can interact with other receptors such as CD27, CD30, and the lymphotoxin-beta receptor, further underscoring its role in diverse signaling pathways. The presence of two alternatively spliced transcript variants encoding the same protein adds complexity to its functional repertoire, while the structural characteristics of TRAF proteins, including their cystine-rich extracellular domains, are vital for their interactions and signaling capabilities.

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TRAF5 Antibody (E-4) References:

1. Targeted disruption of Traf5 gene causes defects in CD40- and CD27-mediated lymphocyte activation.  |  Nakano, H., et al. 1999. Proc Natl Acad Sci U S A. 96: 9803-8. PMID: 10449775
2. Critical roles of TRAF2 and TRAF5 in tumor necrosis factor-induced NF-kappa B activation and protection from cell death.  |  Tada, K., et al. 2001. J Biol Chem. 276: 36530-4. PMID: 11479302
3. Cytoplasmic aggregation of TRAF2 and TRAF5 proteins in the Hodgkin-Reed-Sternberg cells.  |  Horie, R., et al. 2002. Am J Pathol. 160: 1647-54. PMID: 12000717
4. TRAF5 functions in both RANKL- and TNFalpha-induced osteoclastogenesis.  |  Kanazawa, K., et al. 2003. J Bone Miner Res. 18: 443-50. PMID: 12619928
5. Tumor necrosis factor-alpha-induced IKK phosphorylation of NF-kappaB p65 on serine 536 is mediated through the TRAF2, TRAF5, and TAK1 signaling pathway.  |  Sakurai, H., et al. 2003. J Biol Chem. 278: 36916-23. PMID: 12842894
6. Genetic Deficiency of TRAF5 Promotes Adipose Tissue Inflammation and Aggravates Diet-Induced Obesity in Mice.  |  Gissler, MC., et al. 2021. Arterioscler Thromb Vasc Biol. 41: 2563-2574. PMID: 34348490
7. TRAF5 regulates intestinal mucosal Th1/Th17 cell immune responses via Runx1 in colitis mice.  |  Li, M., et al. 2023. Immunology. 170: 495-509. PMID: 37575027
8. CD36 deletion prevents white matter injury by modulating microglia polarization through the Traf5-MAPK signal pathway.  |  Hou, X., et al. 2024. J Neuroinflammation. 21: 148. PMID: 38840180
9. TRAF5, an activator of NF-kappaB and putative signal transducer for the lymphotoxin-beta receptor.  |  Nakano, H., et al. 1996. J Biol Chem. 271: 14661-4. PMID: 8663299
10. TRAF5, a novel tumor necrosis factor receptor-associated factor family protein, mediates CD40 signaling.  |  Ishida, TK., et al. 1996. Proc Natl Acad Sci U S A. 93: 9437-42. PMID: 8790348
11. Human TNF receptor-associated factor 5 (TRAF5): cDNA cloning, expression and assignment of the TRAF5 gene to chromosome 1q32.  |  Nakano, H., et al. 1997. Genomics. 42: 26-32. PMID: 9177772
12. Cloning and characterization of a cDNA encoding the human homolog of tumor necrosis factor receptor-associated factor 5 (TRAF5).  |  Mizushima, S., et al. 1998. Gene. 207: 135-40. PMID: 9511754

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TRAF5 Antibody (E-4) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-TRAF5 Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TRAF5 Antibody (E-4) Specifications

Alternate Product Name: TRAF5

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-257

Epitope Target: TRAF5

Epitope Species: human

Product Reactivity/Detection: TRAF5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for TRAF5 Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin