TRAF2 (h4): 293T Lysate: sc-116412

TRAF2 (h4): 293T Lysate is derived from the human embryonic kidney 293T cell line, genetically engineered to express human TNF Receptor-Associated Factor 2 (TRAF2). TRAF2 is a critical adaptor protein in the TNF receptor signaling pathway, playing a significant role in regulating immune responses, inflammation, and apoptosis. The 293T cell line is selected for its high transfection efficiency and robust protein expression, making it an ideal system for producing TRAF2 lysates. In research, TRAF2 (h4): 293T Lysate is utilized to study the molecular mechanisms of signal transduction initiated by TNF receptor engagement. TRAF2 functions by binding to the cytoplasmic domains of TNF receptors, recruiting additional signaling molecules such as RIP1 and TAK1. This interaction leads to the activation of downstream pathways, including the NF-κB and JNK pathways, which are essential for the transcription of genes involved in inflammatory responses and cell survival. Researchers use TRAF2 (h4): 293T Lysate in various biochemical assays, such as Western blotting, immunoprecipitation, and pull-down assays, to investigate TRAF2 expression, post-translational modifications, and its interactions with other signaling proteins. Additionally, this lysate is employed in functional assays to explore the role of TRAF2 in regulating immune responses and apoptosis.