Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TR4 CRISPR/Cas9 KO Plasmid (m): sc-423490
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TR4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TR4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TR4 Antibody (D-5): sc-365895
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TR4 CRISPR/Cas9 KO Plasmid (m) | sc-423490 | 20 µg | $397.00 |
Overview
Nr2c2 encodes the orphan nuclear receptor TR4 (testicular receptor 4), a ligand-independent transcription factor that binds hormone response elements to regulate gene programs involved in development, metabolism, and cell fate decisions. In mouse systems, TR4 integrates with nuclear receptor signaling networks and influences transcriptional control of lipid and glucose homeostasis, mitochondrial function, and differentiation processes in reproductive, neural, and immune-related contexts. TR4 activity has been linked to modulation of inflammatory signaling and cellular stress responses through broad transcriptional effects, making Nr2c2 a useful node for studying pathway cross-talk. Dysregulation of TR4-associated gene expression has been investigated in models of metabolic dysfunction, neurobiology, and oncogenic phenotypes, supporting its relevance for mechanistic research without implying clinical outcomes.
TR4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nr2c2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nr2c2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nr2c2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TR4 protein expression.
This CRISPR knockout system enables efficient generation of Nr2c2-deficient cell models for investigation of TR4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nr2c2 exon(s) critical for TR4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nr2c2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TR4 CRISPR/Cas9 KO Plasmid (m) and TR4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nr2c2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TR4 HDR Plasmid (m) and TR4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nr2c2 homology arms to support homology-directed repair at defined Nr2c2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.