TPX2 Antibody (B-5): sc-376812

TPX2 Antibody (B-5) is a mouse monoclonal IgM antibody that detects TPX2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TPX2, or targeting protein for Xklp2, is a crucial microtubule-associated protein that plays a significant role in the assembly of microtubules during cell division, particularly in the M phase of the cell cycle. TPX2 is activated by Ran-GTP, which facilitates chromatin-induced microtubule assembly, ensuring proper spindle formation and function. The structural integrity of TPX2 is vital as TPX2 interacts with Aurora-A kinase at the spindle apparatus, where TPX2 may be regulated through phosphorylation, further emphasizing TPX2′s importance in mitotic spindle assembly. Additionally, TPX2 activates Eg2, a kinase that is essential for microtubule dynamics, by enhancing Eg2′s phosphorylation and activity in a microtubule-dependent manner. Notably, TPX2′s function can be inhibited by binding to importin α, a nuclear import factor, which highlights the regulatory mechanisms that control TPX2′s activity. Disruption of TPX2 expression through RNA interference has been shown to lead to significant defects in microtubule organization during mitosis, underscoring TPX2′s critical role in maintaining cellular integrity during cell division.

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TPX2 Antibody (B-5) References:

1. The Aurora-A/TPX2 complex: a novel oncogenic holoenzyme?  |  Asteriti, IA., et al. 2010. Biochim Biophys Acta. 1806: 230-9. PMID: 20708655
2. TPX2 regulates neuronal morphology through kinesin-5 interaction.  |  Kahn, OI., et al. 2015. Cytoskeleton (Hoboken). 72: 340-8. PMID: 26257190
3. TPX2-LIKE PROTEIN3 Is the Primary Activator of α-Aurora Kinases and Is Essential for Embryogenesis.  |  Boruc, J., et al. 2019. Plant Physiol. 180: 1389-1405. PMID: 31097675
4. TPX2 silencing exerts anti‑tumor effects on hepatocellular carcinoma by regulating the PI3K/AKT signaling pathway.  |  Huang, DH., et al. 2019. Int J Mol Med. 44: 2113-2122. PMID: 31638175
5. TPX2 mediates prostate cancer epithelial-mesenchymal transition through CDK1 regulated phosphorylation of ERK/GSK3β/SNAIL pathway.  |  Zhang, B., et al. 2021. Biochem Biophys Res Commun. 546: 1-6. PMID: 33556637
6. Downregulation of TPX2 impairs the antitumor activity of CD8+ T cells in hepatocellular carcinoma.  |  Wang, X., et al. 2022. Cell Death Dis. 13: 223. PMID: 35273149
7. TPX2 enhances the transcription factor activation of PXR and enhances the resistance of hepatocellular carcinoma cells to antitumor drugs.  |  Wang, H., et al. 2023. Cell Death Dis. 14: 64. PMID: 36707511
8. TPX2 expression as a negative predictor of gemcitabine efficacy in pancreatic cancer.  |  Guenther, M., et al. 2023. Br J Cancer. 129: 175-182. PMID: 37142730
9. TPX2 overexpression promotes sensitivity to dasatinib in breast cancer by activating YAP transcriptional signaling.  |  Marugán, C., et al. 2024. Mol Oncol. 18: 1531-1551. PMID: 38357786
10. AurkA/TPX2 co-overexpression in nontransformed cells promotes genome instability through induction of chromosome mis-segregation and attenuation of the p53 signalling pathway.  |  Naso, FD., et al. 2024. Biochim Biophys Acta Mol Basis Dis. 1870: 167116. PMID: 38447882

The preferred TPX2 antibody is TPX2 Antibody (E-2): sc-271570.