TPST-2 CRISPR/Cas9 KO Plasmid (m): sc-423487
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TPST-2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TPST-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TPST-2 CRISPR/Cas9 KO Plasmid (m) | sc-423487 | 20 µg | $397.00 |
Overview
Tpst2 encodes tyrosylprotein sulfotransferase 2 (TPST-2), a Golgi-resident enzyme that catalyzes post-translational tyrosine O-sulfation of secreted and membrane proteins using PAPS as the sulfate donor. This modification shapes protein–protein interactions and receptor–ligand recognition, influencing chemokine signaling, leukocyte trafficking, and other extracellular communication processes. TPST-2 activity contributes to proper maturation and function of multiple peptide hormones and adhesion molecules, linking Tpst2-dependent sulfation to immune and endocrine homeostasis. Dysregulation of tyrosine sulfation has been associated with altered inflammatory signaling and reproductive phenotypes, making Tpst2 a useful node for studying sulfation-dependent signaling networks in mouse models.
TPST-2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tpst2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tpst2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tpst2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TPST-2 protein expression.
This CRISPR knockout system enables efficient generation of Tpst2-deficient cell models for investigation of TPST-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tpst2 exon(s) critical for TPST-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tpst2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TPST-2 CRISPR/Cas9 KO Plasmid (m) and TPST-2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tpst2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TPST-2 HDR Plasmid (m) and TPST-2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tpst2 homology arms to support homology-directed repair at defined Tpst2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.